PXD044848

PXD044848 is an original dataset announced via ProteomeXchange.

Title	Activity-Based Metaproteomics Driven Discovery and Enzymological Characterization of Novel α-Galactosidases in the Gut Microbiome
Description	enzymes. However, many genes in the microbiome remain uncharacterized due to the challenge in culturing intestinal strains in vitro, which limits the identification of target enzymes. To address this issue, we developed an effective Activity-Based MetaProteomics (ABMP) strategy using a specific activity-based probe (ABP) to screen the entire gut microbiome for target enzymes, without the need to isolate and culture bacterial strains individually. α-Galactosidases (AGALs) are widely distributed in gut microbiota, plant, and animal kingdoms of life and have multiple applications in industries such as food, animal feed, and biomedical sectors. Despite the rich source of AGALs in the gut microbiome, many AGAL genes lack functional annotations. Using an activity-based cyclophellitol aziridine probe specific to AGAL, we successfully identified and characterized several new enzymes possessing AGAL activities from the gut microbiome.
HostingRepository	iProX
AnnounceDate	2023-08-25
AnnouncementXML	Submission_2023-08-25_02:30:24.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Penga Peng
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 02:30:04	ID requested
⏵ 1	2023-08-25 02:30:25	announced

Dataset with its publication pending

submitter keyword: Mus musculus, Metaproteomics, activity-based probe, α-Galactosidases,Gut Microbiome

Nan Li
contact affiliation	Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
contact email	nan.li@siat.ac.cn
lab head
Penga Peng
contact affiliation	Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
contact email	hp.peng@siat.ac.cn
dataset submitter

iProX dataset URI