PXD044834

PXD044834 is an original dataset announced via ProteomeXchange.

Title	Cellular Targets and the Lysine Selectivity of the HERC5 ISG15 Ligase
Description	ISG15 is a type I interferon-induced ubiquitin-like protein modifier that functions in innateimmune responses. The single major human ISG15 ligase is HERC5 (hHERC5), a ribosome-associated HECT E3s that broadly ISGylates proteins cotranslationally. Here, we characterized the hHERC5-dependent ISGylome and identified over 5,000 modified lysines in over 2,000 proteins. In parallel, we characterized the substrate selectivity of the major mouse ISG15 ligase, mHERC6, and analysis of sequences surrounding ISGylation sites revealed that hHERC5 and mHERC6 have distinct preferences for amino acid sequence context. Several features of the datasets were consistent with ISGylation of ribosome-tethered nascent chains, and mHERC6, like hHERC5, associated with polysomes and cotranslationally modified nascent polypeptides. Like many genes encoding innate immune effectors, the hHERC5 and mHERC6 genes are under positive genetic selection and we propose that the differing lysine selectivities of these ligases may reflect species-specific adaptations to pathogen challenges experienced over evolutionary timeframes.
HostingRepository	PRIDE
AnnounceDate	2024-03-05
AnnouncementXML	Submission_2024-03-05_09:15:11.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Faull
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-24 14:00:39	ID requested
⏵ 1	2024-03-05 09:15:11	announced

10.1016/j.isci.2024.108820;

Zhao X, Perez JM, Faull PA, Chan C, Munting FW, Canadeo LA, Cenik C, Huibregtse JM, Cellular targets and lysine selectivity of the HERC5 ISG15 ligase. iScience, 27(2):108820(2024) [pubmed]

submitter keyword: innate immunity, post-translational modifications, HERC5, Type I Interferon,ISG15, HERC6, ubiquitin-like proteins, HECT E3

Jon M Huibregtse
contact affiliation	Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712
contact email	huibregtse@austin.utexas.edu
lab head
Peter Faull
contact affiliation	The University of Texas at Austin
contact email	peter.faull@austin.utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD044834

PRIDE project URI

• PRIDE
  1. PXD044834
     1. Label: PRIDE project
     2. Name: Cellular Targets and the Lysine Selectivity of the HERC5 ISG15 Ligase