PXD044831

PXD044831 is an original dataset announced via ProteomeXchange.

Title	Quantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F2 R1 on QE-HF)
Description	O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discovery in wheat revealed an atypical TaOGT(TaOGT1) with no structural similarity to SEC and SPY enzyme (Fan et al. 2021). TaOGT1 was found to O-GlcNAcylate TaGRP2. In Arabidopsis, approximately 34 unannotated or uncharacterized proteins share similarity with TaOGT1, leading to questions about whether SEC is the primary contributor to O-GlcNAcylation in plants. Here we use LWAC enrichment and SILIA labeling, quantifying at both MS1. Our findings reveal a significant reduction in O-GlcNAc levels in the sec mutant, indicating SEC’s critical role in mediating O-GlcNAcylation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:41:09.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-24 09:56:49	ID requested
1	2024-05-22 02:50:39	announced
⏵ 2	2024-10-22 06:41:12	announced	2024-10-22: Updated project metadata.

10.1016/j.mcpro.2024.100732;

Shrestha R, Karunadasa S, Grismer TS, Reyes AV, Xu SL, SECRET AGENT O-GlcNAcylates Hundreds of Proteins Involved in Diverse Cellular Processes in Arabidopsis. Mol Cell Proteomics, 23(4):100732(2024) [pubmed]

submitter keyword: mass spectrometry,O-GlcNAcylation, proteomics., Lectin weak affinity chromatography (LWAC)

SHOULING XU
contact affiliation	CARNEGIE MS FACILITY
contact email	SXU@CIW.EDU
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044831

PRIDE project URI

• PRIDE
  1. PXD044831
     1. Label: PRIDE project
     2. Name: Quantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F2 R1 on QE-HF)