PXD044817

PXD044817 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The interactive proteins of G6PD immunoprecipitated via anti-flag beads in Huh7 cells
Description	Oxaliplatin-based therapeutics is a widely used treatment approach for hepatocellular carcinoma (HCC) patients; however, drug resistance poses a significant clinical challenge. Epigenetic modifications have been implicated in the development of drug resistance. In our study, employing siRNA library screening, we identified that silencing the m6A writer METTL3 significantly enhanced the sensitivity to oxaliplatin in both in vivo and in vitro HCC models. Further investigations through combined RNA-seq and non-targeted metabolomics analysis revealed that silencing METTL3 impeded the pentose phosphate pathway (PPP). Mechanistically, METTL3 was found to regulate G6PD, the rate-limiting enzyme of PPP through the ubiquitination-proteasome pathway. To identify the potential E3-liagese of G6PD, we performed co-IP in G6PD-flag exogenous expression Huh7 cells with anti-flag magnetic beads. The enriched protein sample was subjected for MS identification.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_11:38:16.511.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044817
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yongrui Lv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-23 21:14:59	ID requested
⏵ 1	2025-05-06 11:38:17	announced

Publication List

Jin X, Lv Y, Bie F, Duan J, Ma C, Dai M, Chen J, Lu L, Xu S, Zhou J, Li S, Bi J, Wang F, Xie D, Cai M, METTL3 confers oxaliplatin resistance through the activation of G6PD-enhanced pentose phosphate pathway in hepatocellular carcinoma. Cell Death Differ, 32(3):466-479(2025) [pubmed]
10.1038/s41418-024-01406-2;
10.6019/PXD044817;

Jin X, Lv Y, Bie F, Duan J, Ma C, Dai M, Chen J, Lu L, Xu S, Zhou J, Li S, Bi J, Wang F, Xie D, Cai M, METTL3 confers oxaliplatin resistance through the activation of G6PD-enhanced pentose phosphate pathway in hepatocellular carcinoma. Cell Death Differ, 32(3):466-479(2025) [pubmed]

10.1038/s41418-024-01406-2;

10.6019/PXD044817;

Keyword List

submitter keyword: LC-MSMS, Hepatocellular carcinoma,human, cell line

submitter keyword: LC-MSMS, Hepatocellular carcinoma,human, cell line

Contact List

Muyan Cai
contact affiliation	Sun Yat-sen University Cancer Center, Department of Pathology
contact email	caimy@sysucc.org.cn
lab head
Yongrui Lv
contact affiliation	Sun Yat-sen University Cancer Center
contact email	lvyr@sysucc.org.cn
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD044817

PRIDE project URI

Repository Record List

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