PXD044816
PXD044816 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PML::RARA and GATA2 proteins interact via DNA templates to induce aberrant self-renewal in hematopoietic cells |
| Description | The global protein interactions of the oncofusion protein PML::RARA were identified using proximity labeling followed by mass spectrometry. Briefly, Lineage-depleted bone marrow cells from C57Bl/6 mice were transduced with MSCV-IRES-GFP retroviruses containing an enhanced biotin ligase (TurboID) fused to either the N-terminus or C-terminus of PML::RARAWT (TurboID-PML::RARAWT and PML::RARAWT-TurboID respectively), TurboID-PML::RARAC88A (mutation in the DNA binding domain of RARA), or a TurboID cDNA alone. A flexible Glycine/Serine linker placed between TurboID and PML::RARA allows TurboID to freely rotate, and biotinylate proteins within 10 angstroms of the TurboID-PML::RARA fusion protein. Four days following the first round of transduction, cells were flow sorted on GFP+ cells. Two days following the sort, 1-5 million cells were treated with 50 uM Biotin for four hours at 37 degrees C. Cell lysates were prepared, sonicated, and incubated with streptavidin agarose beads overnight. Beads were washed, peptides were eluted and digested, and then run on a timsTOF Pro 2. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-05 |
| AnnouncementXML | Submission_2024-04-05_11:29:51.993.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jim Malone |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Xlink:DTSSP[88]; Deamidated; Gln->pyro-Glu; Oxidation |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-08-23 20:20:45 | ID requested | |
| ⏵ 1 | 2024-04-05 11:29:52 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: PML::RARA, TurboID, Proximity labeling, Biotin ligase, mass spectrometry |
Contact List
| Timothy J. Ley | |
|---|---|
| contact affiliation | Washington University in St. Louis |
| contact email | timley@wustl.edu |
| lab head | |
| Jim Malone | |
| contact affiliation | Washington University Sch |
| contact email | jmalone@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092739/ |
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