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PXD044765

PXD044765 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBCL2L13 regulates sarcoplasmic reticulum-mitochondria calcium flux in skeletal muscle
DescriptionThe physical connection between mitochondria and endoplasmic or sarcoplasmic reticulum is an essential signaling hub to ensure organelle and cellular functions. In skeletal muscle, ER/SR-mitochondria calcium (Ca2+) signaling is crucial to maintain cellular homeostasis during physical activity. High expression of BCL2L13, a member of the BCL-2 family, was suggested as an adaptive response in endurance-trained human subjects. The aim of this study was to describe the molecular and physiological functions of BCL2L13. Using a zebrafish knockout model, we assessed the physiological changes, alterations of skeletal muscle structure, differences in the muscle proteome, and mitochondrial metabolism caused by the loss of Bcl2l13. We used cellular models to define the subcellular location and the mechanistic role of Bcl2l13. The loss of Bcl2l13 in zebrafish decreased swimming capacity and activity. Skeletal muscle fast fiber cross sectional area was reduced in knockout fish. Muscle proteome uncovered changes in protein turnover, transmembrane transport and expression of Ca2+ signaling proteins compared to wild type fish.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:51:24.379.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterManfredo Quadroni
SpeciesList scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-23 01:22:21ID requested
12024-07-25 09:48:37announced
22024-10-22 06:51:24announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.ISCI.2024.110510;
Keyword List
submitter keyword: Endoplasmic reticulum mitochondria contact sites ERMCs, Mitochondria-associated membranes MAMs, C2C12, Bcl-2 family, Mitochondria-ER contact sites MERCs, zebrafish
Contact List
Francesca Amati
contact affiliationAging and Muscle Metabolism Lab, Department of Biomedical Sciences & Service of Endocrinology, Diabetes and Metabolism, Faculty of Biology and Medicine, Lausanne University Hospital and University of Lausanne, Bugnon 7, 1005 Lausanne, Switzerland
contact emailFrancesca.amati@unil.ch
lab head
Manfredo Quadroni
contact affiliationUniversity of Lausanne
contact emailmanfredo.quadroni@unil.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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