PXD044764

PXD044764 is an original dataset announced via ProteomeXchange.

Title	Synaptotagmin-11 facilitates transport and stabilization of pre-assembled presynaptic GABAB receptor/Cav2.2 signaling complexes
Description	GABAB receptors (GBRs), the G protein-coupled receptors for GABA, regulate synaptic transmission throughout the brain. A main synaptic GBR function is the gating of ion channels. However, where stable GBR-effector channel signaling units are formed in the biosynthetic pathway has remained unclear. Here we show that the vesicular protein synaptotagmin-11 (Syt11) binds the auxiliary GBR subunit KCTD16 and Cav2.2 channels. This enables Syt11 to recruit GBR/Cav2.2 channel complexes to post-Golgi vesicles that transport the pre-assembled signaling complexes in axons and dendrites. Bimolecular fluorescence complementation experiments reveal that GBR/Syt11 complexes are delivered to synaptic sites. Furthermore, Syt11 stabilizes GBRs and Cav2.2 channels at the neuronal plasma membrane by inhibiting constitutive internalization. Syt11-deficient neurons exhibit reduced glutamatergic synaptic transmission and impaired GBR-mediated presynaptic inhibition, thus highlighting a key role for Syt11 in the transport and stable expression of functional GBR/Cav2.2 complexes at synapses.
HostingRepository	PRIDE
AnnounceDate	2024-04-10
AnnouncementXML	Submission_2024-04-10_05:08:02.448.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044764
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jochen Schwenk
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-23 01:17:02	ID requested
⏵ 1	2024-04-10 05:08:02	announced

10.6019/PXD044764;

submitter keyword: LC-MS/MS, interactomics

Prof. Dr. Bernd Fakler
contact affiliation	Institute of Physiology II, University of Freiburg Germany
contact email	bernd.fakler@physiologie.uni-freiburg.de
lab head
Jochen Schwenk
contact affiliation	Institute of Physiology II, University of Freiburg
contact email	jochen.schwenk@physiologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044764
     1. Label: PRIDE project
     2. Name: Synaptotagmin-11 facilitates transport and stabilization of pre-assembled presynaptic GABAB receptor/Cav2.2 signaling complexes