PXD044753

PXD044753 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DNA pulldown of PRDM16 promoter and measurement of LC/MS-MS
Description	To further investigate the transcription cofactor of Smad3 that regulated the transcription of PRDM16, we conducted DNA pull-down assay. we treated HK-2 cells with TGF-β and extracted the nuclear proteins. And the nuclear proteins were incubated with streptavidin magnetic beads, and biotin-labeled DNA probes which bound to the promoter of PRDM16 specifically. After repeatedly washing, the pull-down proteins were analyzed by LC-MS/MS proteomics.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_19:26:31.379.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ben Tang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-22 19:34:11	ID requested
⏵ 1	2025-07-13 19:26:31	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: HK-2 CELL LINE DNA PULL-DOWN LC/MS-MS

submitter keyword: HK-2 CELL LINE DNA PULL-DOWN LC/MS-MS

Contact List

Chun Zhang
contact affiliation	Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
contact email	drzhangchun@hust.edu.cn
lab head
Ben Tang
contact affiliation	Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
contact email	tangbenhust@126.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD044753
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD044753

PRIDE project URI

Repository Record List

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