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PXD044732

PXD044732 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCDK activity at the centrosome regulates the cell cycle
DescriptionCyclin-dependent kinases (CDKs) complexed with cyclins drive progression through the eukaryotic cell cycle. From yeast to human cells, cyclin-CDK localises to the centrosome, but the importance of this localisation is unclear. The conserved ‘hydrophobic patch’ substrate docking site on human Cyclin B1 and on the equivalent fission yeast Cdc13 mediates their localisation to the centrosome and the spindle-pole body (SPB, yeast centrosome equivalent). A hydrophobic patch mutant (HPM) of Cdc13 cannot enter mitosis, but whether this mitotic defect is due to defective SPB localisation or defective cyclin-substrate docking is unknown. Here we show that artificially restoring Cdc13HPM SPB localisation in fission yeast partially rescues both mitosis and defective CDK substrate phosphorylation at both the SPB and within the cytoplasm. In addition, we found that an HPM of the S-phase cyclin Cig2 has defective SPB localisation but is still able to perform bulk DNA synthesis. Our results demonstrate that the hydrophobic patch mediates the SPB localisation of both S- and M-phase cyclins, and that Cdc13 SPB localisation is essential for mitotic entry and for full phosphorylation of CDK substrates, supporting the view that the centrosome plays a role as a signalling hub regulating CDK cell cycle control.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:35:09.161.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTania Auchynnikava
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationListphosphorylated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-22 04:16:57ID requested
12024-04-04 02:49:49announced
22024-10-22 06:35:09announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.CELREP.2024.114066;
Keyword List
submitter keyword: Cell Cycle, CDK, phosphrylation
Contact List
Paul Nurse
contact affiliationThe Francis Crick Institute
contact emailpaul.nurse@crick.ac.uk
lab head
Tania Auchynnikava
contact affiliationThe Francis Crick Institute
contact emailauchynt@crick.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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