PXD044711

PXD044711 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Anxiety Complicated by Acute Myocardial Infarction: LMSXF Intervention in Rats
Description	BACKGROUND: Anxiety is highly prevalent in patients with acute myocardial infarction (AMI ), significantly impacting their quality of life. This study delved into the path mechanism of anxiety in comorbid AMI using proteomics technology, alongside behavioral and neurotransmitter assessments. The study also explored the potential myocardial protective and anxiolytic effects of LongmuShuangxinfang (LMSXF), a traditional Chinese herbal formula. METHODS: An experimental model was established by ligating the left anterior descending coronary artery and employing unpredictable empty-bottle stimulation in 18 rats divided into sham, model, and traditional Chinese medicine (TCM) groups. Proteomic analyses were conducted using label-free technology on the hippocampus and myocardial infarction marginal tissues. Parallel Reaction Monitoring (PRM) verified proteomic results and identified differential proteins. RESULTS: Proteomic and bioinformatics analyses revealed that exacerbation of anxiety symptoms in AMI might be linked to processes like the neurotransmitter receptor metabolic process, cellular response to oxidative stress, and reduced hippocampal hemoglobin levels. Myocardial injury was associated with responses to ischemia, inflammation, and calcium ion homeostasis. PRM validation highlighted the crucial roles of proteins including Gnb1, Ahsa1, Psmd14, Frrs1l, Cfl1, Aldh, and Stub1 in MI-anxiety pathogenesis. LMSXF enhanced neurotransmitter levels and anxiety status and mitigated myocardial injury by influencing hemoglobin complex levels, oxidant detoxification, neuropeptide signaling, and postsynaptic modulation.
HostingRepository	iProX
AnnounceDate	2023-08-22
AnnouncementXML	Submission_2024-06-02_20:01:00.010.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weizhe Zhao
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 18:57:21	ID requested
⏵ 1	2024-06-02 20:01:00	announced

Dataset with its publication pending

submitter keyword: LongmuShuangxinFang

anxiety

acute myocardial infarction

traditional Chinese medicine

Proteomics

Jiqiu Hou
contact affiliation	Beijing University of Chinese Medicine
contact email	houjiqiu1992@163.com
lab head
Weizhe Zhao
contact affiliation	Beijing University of Chinese Medicine
contact email	zhaoweizhe@126.com
dataset submitter

iProX dataset URI