PXD044708

PXD044708 is an original dataset announced via ProteomeXchange.

Title	Proteomic Characterization and Antiproliferative Potential of Proteins from Cuscuta Epithymum Crude Herbal Product Against MCF-7 Human Breast Cancer Cell Line
Description	Phytochemicals from Cuscuta have been reported for exhibiting anticancer properties; however, the bioactivities attributed to the proteins remained unexplored. This study examined the proteins from a crude herbal product of Cuscuta epithymum (CE) harvested from host plants Alhaji maurorum and Medicago sativa for inhibiting the proliferation of the MCF-7 breast cancer cell line. The CE crude proteins (CP) were separated into four protein peaks (P1, P2, P3, and P4) by gel filtration chromatography. CP, P1, P2, and P3 showed dose-dependent growth inhibitory response in the MTT assay with respective IC50 values of 33.8, 43.1, 34.5, and 28.6 µg/ml. In western-blot analysis, upregulation of BCL2-associated X protein (BAX) with concomitant downregulation of BCL-2 in the cells treated with crude and P3 was observed. In cells treated with P2, BAX was upregulated; however, no significant change in the expression of BCL-2 was recorded. The LC-MS/MS analysis revealed the identity of those proteins in CE, such as peroxidase, non-specific lipid transfer protein, and superoxide dismutase, etc., which have been previously reported from other plants for exhibiting anticancer activity. The findings indicated that Cuscuta proteins strongly inhibited the proliferation of breast cancer MCF-7 cells by apoptosis. The survey of the CE protein profile suggests it as an enriched source of proteins with high biological significance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:02:06.470.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044708
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	basir syed
SpeciesList	scientific name: Cuscuteae; NCBI TaxID: 292919;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 15:26:05	ID requested
1	2023-08-29 09:05:33	announced
⏵ 2	2024-10-22 06:02:06	announced	2024-10-22: Updated project metadata.

10.6019/PXD044708;

submitter keyword: Protein Fractions, Gel filtration,Cuscuta, Anti-Proliferative_MCF-7

basir syed
contact affiliation	Chapman University School of Pharmacy
contact email	bsyed@chapman.edu
lab head
basir syed
contact affiliation	chapman University school of pharmacy
contact email	bsyed@chapman.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044708
     1. Label: PRIDE project
     2. Name: Proteomic Characterization and Antiproliferative Potential of Proteins from Cuscuta Epithymum Crude Herbal Product Against MCF-7 Human Breast Cancer Cell Line