PXD044685

PXD044685 is an original dataset announced via ProteomeXchange.

Title	Myosin PTM peptide mapping from hibernating and active bears and squirrels
Description	Recent discoveries in the field of skeletal muscle have found that the contractile protein myosin is able to dictate the resting metabolic rate of this organ. In this study we aimed to investigate if myosin is involved in the process of metabolic shutdown which is observed during hibernation. Comparing large and small hibernating mammals, we found that in small hibernating mammals, the rate of ATP turnover dictated by myosin was altered and that this was also dependent upon the temperature of skeletal muscle. We further investigated changes to the whole proteome of these animals and observed that in small hibernators found significant changes to sarcomere organization during hibernating periods. Finally, we identified hyperphosphorylation upon the myosin molecule in these small hibernators which was predicted to induce stability changes to this molecule. In this dataset, we performed PTM peptide mapping of the myosin protein derived from skeletal muscle fibre samples from squirrels collected in summer and winter time.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:37.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Spermophilus tridecemlineatus (Thirteen-lined ground squirrel); NCBI TaxID: 43179; scientific name: Ursus arctos (Brown bear) (Grizzly bear); NCBI TaxID: 9644;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 03:47:43	ID requested
1	2024-04-29 02:54:21	announced
⏵ 2	2024-10-22 06:37:37	announced	2024-10-22: Updated project metadata.

10.7554/ELIFE.94616.1;

submitter keyword: Muscle, Myosin, Hibernation

Julien Ochala
contact affiliation	University of Copenhagen Department of Biomedical Sciences Blegdamsvej 3B København N, DK-2200
contact email	julien.ochala@sund.ku.dk
lab head
Michael Wierer
contact affiliation	Proteomics Research Infrastructure, University of Copenhagen
contact email	michael.wierer@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD044685

PRIDE project URI

• PRIDE
  1. PXD044685
     1. Label: PRIDE project
     2. Name: Myosin PTM peptide mapping from hibernating and active bears and squirrels