PXD044683

PXD044683 is an original dataset announced via ProteomeXchange.

Title	1. SILAC based secretome analysis of WT and tKO cells
Description	Integrins play a vital role in coordinating between the extracellular matrix (ECM) and the intracellular environment, thus enabling transduction of biomechanical signals to maintain tissue homeostasis. Here we investigate the significance of collagen-binding integrins in regulating fibroblast functions with relevance to fibrosis. Our data suggest that secretome from primary dermal fibroblasts isolated from mice lacking three collagen-binding integrins α1, α2 and α11 shows downregulation of several proteins essential for structure and regulation of crucial pro-fibrotic ECM components. In summary, abrogation of integrin-mediated cell-collagen association attenuates fibrosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:00:54.993.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prerana Wagle
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 02:29:49	ID requested
1	2023-08-21 02:39:26	announced
⏵ 2	2024-10-22 06:00:55	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Collagen,Integrin, Fibrosis, Fibroblast

Thomas Krieg
contact affiliation	Translational Matrix Biology, University of Cologne, Medical Faculty, Cologne Cologne Excellence Cluster on Cellular Stress Responses in Ageing-Associated Diseases (CECAD)and Center for Molecular Medicine (CMMC), University of Cologne, Cologne, Germany
contact email	thomas.krieg@uni-koeln.de
lab head
Prerana Wagle
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD044683

PRIDE project URI

• PRIDE
  1. PXD044683
     1. Label: PRIDE project
     2. Name: 1. SILAC based secretome analysis of WT and tKO cells