PXD044681

PXD044681 is an original dataset announced via ProteomeXchange.

Title	A NET4-RabG3 couple mediate the link between actin and the tonoplast and is essential for normal actin cytoskeletal remodelling in stomatal closure to flg22
Description	Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal closure in plant immunity. NET4 proteins interact with F-actin and with members of the Rab7 GTPase RABG3 family through two distinct domains, allowing for simultaneous localization to actin filaments and the tonoplast. NET4 proteins interact with GTP-bound, active RABG3 members, suggesting their function being downstream effectors. We also show that RABG3b is critical for stomatal closure induced by microbial patterns. Taken together, we conclude that the actin cytoskeletal remodelling during stomatal closure involves a molecular link between actin filaments and the tonoplast, which is mediated by the NET4-RABG3b interaction. We propose that stomatal closure to microbial patterns involves the coordinated action of immune-triggered osmotic changes and actin cytoskeletal remodelling likely driving compact vacuolar morphologies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:26:32.034.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044681
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominique Eeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 01:30:35	ID requested
1	2023-09-22 01:02:35	announced
⏵ 2	2023-11-14 07:26:32	announced	2023-11-14: Updated project metadata.

10.6019/PXD044681;

submitter keyword: RABG3,NET4, TAP

Patrick Hussey
contact affiliation	Department of Biosciences, University of Durham, South Road, Durham, DH1 3LE, UK
contact email	p.j.hussey@durham.ac.uk
lab head
Dominique Eeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	Dominique.Eeckhout@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD044681

PRIDE project URI

• PRIDE
  1. PXD044681
     1. Label: PRIDE project
     2. Name: A NET4-RabG3 couple mediate the link between actin and the tonoplast and is essential for normal actin cytoskeletal remodelling in stomatal closure to flg22