PXD044671
PXD044671 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SDMA-shotgun analysis in pancreatic cancer cell lines |
| Description | As a classic tumor suppressor pathway, Hippo signaling axis is activated by various extra-pathway factors to regulate cell differentiation and organ development. However, recent studies have reported that the activation of Hippo signaling pathway may be more dependent on the autophosphorylation of its core kinase cassette. Here, we demonstrate that protein arginine methyltransferase 5 (PRMT5) is involved in inducing the inactivation of Hippo signaling pathway in pancreatic cancer. Our study shows that the initiator serine/threonine kinase 3 (STK3, also known as MST2) of Hippo signaling pathway can be symmetrically di-methylated at arginine-461 (R461) and arginine-467 (R467) in the SARAH domain by PRMT5, and the methylated MST2 suppresses its autophosphorylation and kinase activity by blocking the formation of homodimer, thereby inactivating Hippo signaling pathway in pancreatic cancer. Moreover, we also discover that the specific PRMT5 inhibitor GSK3326595 re-activates the dysregulated Hippo signaling pathway |
| HostingRepository | iProX |
| AnnounceDate | 2023-08-18 |
| AnnouncementXML | Submission_2024-02-21_18:41:35.233.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yan Sun |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | dimethylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-08-20 20:19:56 | ID requested | |
| 1 | 2023-08-20 20:20:20 | announced | |
| ⏵ 2 | 2024-02-21 18:41:36 | announced | 2024-02-22: Update Information |
Publication List
| Sun Y, Jin X, Meng J, Guo F, Chen T, Zhao X, Wu H, Ren D, MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression. EMBO J, 42(23):e114558(2023) [pubmed] |
Keyword List
| submitter keyword: PRMT5, MST2, methylation, dimerization, pancreatic cancer |
Contact List
| Heshui Wu | |
|---|---|
| contact affiliation | Wuhan Union Hospital |
| contact email | heshuiwu@hust.edu.cn |
| lab head | |
| Yan Sun | |
| contact affiliation | Wuhan Union Hospital |
| contact email | 957718660@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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