PXD044650

PXD044650 is an original dataset announced via ProteomeXchange.

Title	The COP9 signalosome reduces neuroinflammation and attenuates ischemic neuronal stress in organotypic brain slice culture model
Description	In the context of studying the role of the COP9 signalosome (CSN) in neuroinflammation and ischemic neuronal damage, we studied the effect of the cullin NEDDylation state-modifying drugs MLN4924 and CSN5i-3 in BV2 microglial cells, an immortalized murine cell line featuring many of the characteristics of primary microglia. Owing to its potent inhibitory effect on the NEDDylation cascade, MLN4924 exhibits a CSN5-like anti-inflammatory activity. Csn5i-3 is a small molecule inhibitor that specifically binds to CSN5, while it resides in the CSN holocomplex and blocks its deNEDDylase activity, thus leading to an accumulation of NEDDylated cullins. We performed untargeted mass spectrometry-based proteomics of the cell lysates after treating BV2 cells with these drugs under basal culture stress conditions. The proteomic analysis revealed that MLN4924 and CSN5i-3 substantially altered the microglial proteome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:07:35.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rahul Chakraborty
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 08:44:47	ID requested
1	2023-10-24 08:05:54	announced
2	2023-11-14 09:06:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:07:38	announced	2024-10-22: Updated project metadata.

Tian Y, Milic J, Monasor LS, Chakraborty R, Wang S, Yuan Y, Asare Y, Behrends C, Tahirovic S, Bernhagen J, The COP9 signalosome reduces neuroinflammation and attenuates ischemic neuronal stress in organotypic brain slice culture model. Cell Mol Life Sci, 80(9):262(2023) [pubmed]

10.1007/s00018-023-04911-8;

submitter keyword: Constitutive photomorphogenesis 9 signalosome (CSN), ischemic stroke, JAB1, CSN5, microglia, organoid

Juergen Bernhagen
contact affiliation	Vascular Biology, Institute for Stroke and Dementia Research (ISD), LMU Klinikum, Ludwig-Maximilians-University (LMU) Munich, Germany.
contact email	Juergen.Bernhagen@med.uni-muenchen.de
lab head
Rahul Chakraborty
contact affiliation	Munich Cluster for Systems Neurology (SyNergy), LMU Munich
contact email	Rahul.Chakraborty@mail03.med.uni-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044650
     1. Label: PRIDE project
     2. Name: The COP9 signalosome reduces neuroinflammation and attenuates ischemic neuronal stress in organotypic brain slice culture model