PXD044649

PXD044649 is an original dataset announced via ProteomeXchange.

Title	The low abundance plasma proteome reveals changes associ-ated with breast implant capsular contracture
Description	Capsular contracture (CC) is one of the most common post-operative complication associated with breast-implant associated infections. The mechanisms that lead to CC remain poorly understood. Plasma is an ideal biospecimen for early proteomics biomarker discovery. However, as higher-abundant proteins mask signals from low-abundance proteins, the identification of novel or specific proteins as biomarkers for a particular disease has been hampered. Here, we employed depletion of high-abundance plasma proteins followed by Tandem Mass Tag (TMT)-based quantitative proteomics to compare 10 healthy control patients against 10 breast implant CC patients. A total of 450 proteins were identified from these samples, 43 of which were significantly (p < 0.05) identified in this study. Among them 16 proteins were significantly differentially expressed in which 5 proteins were upregulated and 11 downregulated in breast implant CC patients compared to healthy controls. GO enrichment analysis revealed that proteins related to cell, cellular processes and catalytic activity were highest in the cellular component, biological process, and molecular function categories, respectively. Further, pathway analysis revealed that inflammatory responses, focal adhesion, platelet activation, complement and coagulation cascades were enriched pathways. The differentially expressed proteins from TMT-based quantitative proteomics has the potential to provide important information for future mechanistic studies and in the development of breast implant CC biomarkers
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_03:11:33.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ardeshir Amirkhani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 08:44:08	ID requested
⏵ 1	2024-10-17 03:11:34	announced

Rahman MA, Amirkhani A, Mempin M, Ahn SB, Deva AK, Baker MS, Vickery K, Hu H, The Low-Abundance Plasma Proteome Reveals Differentially Abundant Proteins Associated with Breast Implant Capsular Contracture: A Pilot Study. Proteomes, 12(3):(2024) [pubmed]

10.3390/proteomes12030022;

submitter keyword: proteomics, TMT,Breast implants, capsular contracture, human plasma

Belinda Schiller
contact affiliation	The Australian Proteome Analysis Facility (APAF) is a service and research facility which is part of the Macquarie Analytical and Fabrication Facility (MAFF) within Macquarie University.
contact email	belinda.schiller@mq.edu.au
lab head
Ardeshir Amirkhani
contact affiliation	Macquarie University
contact email	ardeshir.amirkhani@mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044649
     1. Label: PRIDE project
     2. Name: The low abundance plasma proteome reveals changes associ-ated with breast implant capsular contracture