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PXD044649

PXD044649 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe low abundance plasma proteome reveals changes associ-ated with breast implant capsular contracture
DescriptionCapsular contracture (CC) is one of the most common post-operative complication associated with breast-implant associated infections. The mechanisms that lead to CC remain poorly understood. Plasma is an ideal biospecimen for early proteomics biomarker discovery. However, as higher-abundant proteins mask signals from low-abundance proteins, the identification of novel or specific proteins as biomarkers for a particular disease has been hampered. Here, we employed depletion of high-abundance plasma proteins followed by Tandem Mass Tag (TMT)-based quantitative proteomics to compare 10 healthy control patients against 10 breast implant CC patients. A total of 450 proteins were identified from these samples, 43 of which were significantly (p < 0.05) identified in this study. Among them 16 proteins were significantly differentially expressed in which 5 proteins were upregulated and 11 downregulated in breast implant CC patients compared to healthy controls. GO enrichment analysis revealed that proteins related to cell, cellular processes and catalytic activity were highest in the cellular component, biological process, and molecular function categories, respectively. Further, pathway analysis revealed that inflammatory responses, focal adhesion, platelet activation, complement and coagulation cascades were enriched pathways. The differentially expressed proteins from TMT-based quantitative proteomics has the potential to provide important information for future mechanistic studies and in the development of breast implant CC biomarkers
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_03:11:33.941.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterArdeshir Amirkhani
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-18 08:44:08ID requested
12024-10-17 03:11:34announced
Publication List
Rahman MA, Amirkhani A, Mempin M, Ahn SB, Deva AK, Baker MS, Vickery K, Hu H, The Low-Abundance Plasma Proteome Reveals Differentially Abundant Proteins Associated with Breast Implant Capsular Contracture: A Pilot Study. Proteomes, 12(3):(2024) [pubmed]
10.3390/proteomes12030022;
Keyword List
submitter keyword: proteomics, TMT,Breast implants, capsular contracture, human plasma
Contact List
Belinda Schiller
contact affiliationThe Australian Proteome Analysis Facility (APAF) is a service and research facility which is part of the Macquarie Analytical and Fabrication Facility (MAFF) within Macquarie University.
contact emailbelinda.schiller@mq.edu.au
lab head
Ardeshir Amirkhani
contact affiliationMacquarie University
contact emailardeshir.amirkhani@mq.edu.au
dataset submitter
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