PXD044639

PXD044639 is an original dataset announced via ProteomeXchange.

Title	Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig.
Description	The seminal plasma (SP) is the liquid component of semen that facilitates sperm transport through the female genital tract. SP modulates the activity of the ovary, oviductal environment and uterine function during the periovulatory and early pregnancy period. Extracellular vesicles (EVs) secreted in the oviduct (oEVs) and uterus (uEVs) have been shown to influence the expression of endometrial genes that regulate fertilization and early embryo development. In some species, semen is composed of well-separated fractions that vary in concentration of spermatozoa and SP composition and volume. This study aimed to investigate the impact of different accumulative fractions of the porcine ejaculate (F1, composed of the sperm-rich fraction (SRF); F2, composed of F1 plus the intermediate fraction; F3, composed of F2 plus the post-SRF) on oEVs and uEVs protein cargo. Six days after the onset of estrus, we determined the oEVs and uEVs size and protein concentration in pregnant sows by artificial insemination (AI-sows) and in non-inseminated sows as control (C-sows). We also identified the main proteins in oEVs and uEVs, in AI-F1, AI-F2, AI-F3, and C-sows. Our results indicated that although the size of EVs is similar between AI- and C-sows, the protein concentration of both oEVs and uEVs was significantly lower in AI-sows (p < 0.05). Proteomic analysis identified 38 unique proteins in oEVs from AI-sows, mainly involved in protein stabilization, glycolytic and carbohydrate processes. The uEVs from AI-sows showed the presence of 43 unique proteins, including already-known fertility-related proteins (EZR, HSPAA901, PDS). We also demonstrated that the protein composition of oEVs and uEVs differed depending on the seminal fraction(s) inseminated (F1, F2, or F3). In conclusion, we have found a specific protein cargo in uterine and oviductal EVs depending on the type of semen fraction the sow was inseminated with, and these insemination with different seminal fractions results in the oviductal and uterine secretion of specific EVs proteins are closely associated with reproductive processes.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:02:38.002.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Santa María Toledo Guardiola
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue
Instrument	Agilent instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 08:34:28	ID requested
⏵ 1	2024-01-26 06:02:40	announced

Toledo-Guardiola SM, Luongo C, Abril-Parre, ñ, o L, Soriano-, Ú, beda C, Mat, á, s C, Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Front Cell Dev Biol, 11():1231755(2023) [pubmed]

Luongo C, Gonz, á, lez-Brusi L, Cots-Rodr, í, guez P, Izquierdo-Rico MJ, Avil, é, s M, Garc, í, a-V, á, zquez FA, Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site. Int J Mol Sci, 21(17):(2020) [pubmed]

10.3390/biology11020210;

10.3389/fcell.2023.1231755;

Luongo C, Llamas-L, ó, pez PJ, Hern, á, ndez-Caravaca I, Mat, á, s C, Garc, í, a-V, á, zquez FA, Should All Fractions of the Boar Ejaculate Be Prepared for Insemination Rather Than Using the Sperm Rich Only? Biology (Basel), 11(2):(2022) [pubmed]

10.3390/ijms21176060;

submitter keyword: oviductal fluid, uterine fluid., extracellular vesicles, insemination, pregnant sows,Ejaculate fractions

Carmen Matás
contact affiliation	1: Physiology of reproduction, Veterinary Faculty, Campus of Excellence Mare Nostrum, University of Murcia. 2: IMIB-Arrixaca, Murcia, Spain.
contact email	cmatas@um.es
lab head
Santa María Toledo Guardiola
contact affiliation	University of Murcia
contact email	smaria.toledo@um.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD044639

PRIDE project URI

• PRIDE
  1. PXD044639
     1. Label: PRIDE project
     2. Name: Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig.