PXD044629

PXD044629 is an original dataset announced via ProteomeXchange.

Title	hTERT mesenchymal stem cells (MSCs), Stem cells EVs LC-MS/MS
Description	As the economic challenges associated with vision impairment and ocular injuries continue to escalate, there exists an urgent need to explore innovative remedies for these conditions. Extracellular Vesicles (EVs), containing a diverse array of biological components such as nucleic acids, proteins, and lipids, have garnered substantial scientific support for their healing and immune-regulating attributes in addressing various disorders. Nevertheless, an area that remains relatively uncharted is the potential reparative impact of stem cell-derived EVs within the realm of ocular damage or disease. Furthermore, prevailing literature predominantly focuses on EVs derived from primary stem cells, neglecting a comprehensive assessment of EVs derived from hTERT-immortalized stem cells. Through our extensive EV production platform, we have consistently generated EVs sourced from hTERT mesenchymal stem cells (MSCs) and have employed diverse techniques to characterize and profile their cargo contents. Leveraging established cellular assays, we have conducted a comparative analysis of these EVs' effects on both intact and impaired retinal pigment epithelial cells. To the best of our knowledge, this marks the inaugural study demonstrating the feasibility of consistent, large-scale production of hTERT MSC EVs and exploring their potential regenerative properties against compromised ocular cells. The outcomes of our investigations affirm that hTERT MSCs elicit multiple reparative influences in vitro, underscoring their promise not only as a viable substitute for primary MSC-derived EVs but also as a prospective innovative therapeutic agent for addressing ocular injuries.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-15_22:54:43.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fatah Kashanchi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 08:32:27	ID requested
⏵ 1	2024-06-15 22:54:44	announced

Hindle J, Williams A, Kim Y, Kim D, Patil K, Khatkar P, Osgood Q, Nelson C, Routenberg DA, Howard M, Liotta LA, Kashanchi F, Branscome H, hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells. Cells, 13(10):(2024) [pubmed]

10.3390/cells13100861;

submitter keyword: Stem cells, EVs, LC-MS/MS

Fatah Kashanchi
contact affiliation	Professor, Virology Director of the Laboratory of Molecular Virology
contact email	fkashanc@gmu.edu
lab head
Fatah Kashanchi
contact affiliation	George Mason University
contact email	fkashanc@gmu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD044629

PRIDE project URI

• PRIDE
  1. PXD044629
     1. Label: PRIDE project
     2. Name: hTERT mesenchymal stem cells (MSCs), Stem cells EVs LC-MS/MS