PXD044621

PXD044621 is an original dataset announced via ProteomeXchange.

Title	GSG1L-containing AMPA receptors are complexes with unique spatiotemporal expression, subunit composition and gating properties
Description	Transmembrane AMPA receptor regulatory proteins (TARPs) and germ cell-specific gene 1-like protein (GSG1L) are claudin-type AMPA receptor (AMPAR) auxiliary subunits that profoundly regulate glutamatergic synapse strength and plasticity. While AMPAR-TARP complexes have been extensively studied, less is known about the GSG1L-containing AMPAR complex. Here, we show that GSG1L’s spatiotemporal expression, native interactome and allosteric sites are distinct. GSG1L generally expresses late during brain development in a cell-type and region-specific manner, finally constituting about 5% of all AMPAR complexes in the adult brain (at adulthood). While GSG1L can co-assemble with cornichon and TARP subunits into AMPAR complexes, it may also form the sole auxiliary subunit. Unexpectedly, GSG1L acts through two discrete, evolutionarily-conserved sites on the agonist-binding domain with a weak allosteric interaction at the KGK site, shared with TARPs to slow AMPAR desensitization, and a stronger interaction at a novel site that slows recovery from desensitization.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:09:28.792.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044621
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jochen Schwenk
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	S-carboxamidoethyl-L-cysteine; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 05:54:31	ID requested
⏵ 1	2024-01-26 06:09:29	announced

10.1038/s41467-023-42517-7;

Perozzo AM, Schwenk J, Kamalova A, Nakagawa T, Fakler B, Bowie D, GSG1L-containing AMPA receptor complexes are defined by their spatiotemporal expression, native interactome and allosteric sites. Nat Commun, 14(1):6799(2023) [pubmed]

10.6019/PXD044621;

submitter keyword: LC-MS/MS, interactomics

Prof. Dr. Bernd Fakler
contact affiliation	Institute of Physiology II University of Freiburg, Germany
contact email	bernd.fakler@physiologie.uni-freiburg.de
lab head
Jochen Schwenk
contact affiliation	Institute of Physiology II, University of Freiburg
contact email	jochen.schwenk@physiologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044621
     1. Label: PRIDE project
     2. Name: GSG1L-containing AMPA receptors are complexes with unique spatiotemporal expression, subunit composition and gating properties