PXD044597 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spo13/MEIKIN ensures a two-division meiosis by preventing the activation of APC/CAma1 at meiosis I |
Description | Genome haploidization at meiosis depends on two consecutive nuclear divisions, which are controlled by an oscillatory system consisting of Cdk1-cyclin B and the APC/C bound to the Cdc20 activator. How the oscillator generates exactly two divisions has been unclear. We have studied this question in yeast where exit from meiosis involves accumulation of the APC/C activator Ama1 at meiosis II. We show that inactivation of the meiosis I-specific protein Spo13/MEIKIN results in a single-division meiosis due to premature activation of APC/CAma1. In the wild-type, Spo13 bound to the polo-like kinase Cdc5 prevents Ama1 synthesis at meiosis I by stabilizing the translational repressor Rim4. In addition, Cdc5-Spo13 inhibits the activity of Ama1 by converting the B-type cyclin Clb1 from a substrate to an inhibitor of Ama1. Cdc20-dependent degradation of Spo13 at anaphase I unleashes a feedback loop that increases Ama1’s synthesis and activity, leading to irreversible exit from meiosis at the second division. Thus, by repressing the exit machinery at meiosis I, Cdc5-Spo13 ensures that cells undergo two divisions to produce haploid gametes. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-03 |
AnnouncementXML | Submission_2023-09-03_12:38:29.755.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | BarbaraSteigenberger |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-15 16:07:25 | ID requested | |
⏵ 1 | 2023-09-03 12:38:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: APC/C-Ama1 / Cyclin phosphorylation / Meiotic exit / Polo-like kinase / Spo13/MEIKIN / Translational repressor |
Contact List
Dr. WolfgangZachariae |
contact affiliation | Laboratory of Chromosome Biology Max Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried, Germany |
contact email | zachar@biochem.mpg.de |
lab head | |
BarbaraSteigenberger |
contact affiliation | MPI of Biochemistry |
contact email | steigenberger@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044597
- Label: PRIDE project
- Name: Spo13/MEIKIN ensures a two-division meiosis by preventing the activation of APC/CAma1 at meiosis I