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PXD044582

PXD044582 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA CHD8-TRRAP axis facilitates MYC- and E2F-target gene regulation in human neural stem cells.
DescriptionMutations in ATP-dependent chromatin remodeler CHD8 cause one of the most frequent monogenetic forms of autism and also associate with brain overgrowth. Nevertheless, activities of CHD8 in autism-relevant cell types are still poorly understood. Here we purify the CHD8 protein from human neural stem cells and determine its interaction partners by mass spectrometry. We identify the TRRAP-complex, a coactivator of MYC and E2F transcription factors, as a prominent CHD8 interaction partner. CHD8 colocalizes genome-wide with TRRAP and TRRAP and CHD8 bind together to MYC- and E2F-target gene promoters in human neural stem cells. Acute depletion of CHD8 or TRRAP from human neural stem cells shows down-regulation of MYC- and E2F-target genes as most prominent gene-regulatory events. MYC and E2F factors are established oncogenes known to regulate cell growth. Our results link CHD8 to TRRAP in facilitating regulation of MYC- and E2F-target genes in neural stem cells.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_07:46:47.537.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeroen Demmers
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-15 01:30:06ID requested
12025-05-06 07:46:48announced
Publication List
10.1016/j.isci.2025.111978;
Meert L, Pelicano de Almeida M, Dekker MR, Dekkers DHW, Nowosad K, Huylebroeck D, van den Hout M, Ozg, ΓΌ, r Z, van IJcken WFJ, Demmers J, Fornerod M, Poot RA, A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells. iScience, 28(3):111978(2025) [pubmed]
Keyword List
submitter keyword: neural stem cells,CHD8, MYC, gene regulation, EZ2F
Contact List
Jeroen Demmers
contact affiliationProteomics Center, Erasmus MC
contact emailj.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact emailj.demmers@erasmusmc.nl
dataset submitter
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Dataset FTP location
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