PXD044549

PXD044549 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal excision of flg22 epitope from bacterial flagellin
Description	Plants initiate specific defense responses by recognizing conserved epitope peptides within the flagellin proteins derived from pathogenic bacteria. For perception of epitope by the plant receptor, proteolytic cleavage of epitope peptides from flagellin by plant apoplastic proteases is thought to be crucial. However, the identity of the plant proteases involved in this process remains unknown. Here, we established a one-step identification system for the target proteases in Arabidopsis apoplastic fluid by native two-dimensional electrophoresis followed by an in-gel proteolysis assay using a fluorescence-quenching peptide substrate. We designed a substrate to specifically detect proteolytic activity at the C-terminus of flg22 epitope in flagellin and identified two plant subtilases, SBT5.2, and SBT1.7, as specific proteases responsible for the C-terminal excision of flg22. In the apoplastic fluid of Arabidopsis mutant plants deficient in these two proteases, we observed a decrease in C-terminal excision activity of flg22 domain from flagellin, leading to increase in C-terminally longer flg22 epitope fragments. Consequently, defensive ROS production was delayed in sbt5.2 sbt1.7 double mutant leaf disks compared to WT upon flagellin treatment.
HostingRepository	jPOST
AnnounceDate	2024-04-11
AnnouncementXML	Submission_2024-04-13_16:23:18.662.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044549
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sayaka Matsui
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Pseudomonas syringae pv. tomato str. DC3000; NCBI TaxID: 223283; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-14 00:55:48	ID requested
⏵ 1	2024-04-13 16:23:19	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: flagellin digestion, in-gel proteomics

submitter keyword: flagellin digestion, in-gel proteomics

Contact List

Yoshikatsu Matsubayashi
lab head
Sayaka Matsui
contact affiliation	Nagoya University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002286/