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PXD044541

PXD044541 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePTMC_A pyridinium-based strategy for bioorthogonal conjugation-assisted purification method for profiling cell surface proteome
DescriptionCell surface proteins (CSPs) are valuable targets for therapeutic agents, but achieving highly selective CSP enrichment in cellular physiology remains a technical challenge. To address this challenge, we propose a newly developed sulfo-pyridinium-ester (SPE) cross-linking probe, followed by two-step imaging and enrichment. The SPE probe shows higher efficiency in labelling proteins at the level of cell lysates than similar NHS esters and demonstrates specificity for Lys in competitive experiments. More importantly, this probe can selectively label cell membranes in cell imaging, while only negligible labelling of the intracellular compartment was detected. Moreover, we successfully performed this strategy to mainly label the cell surface proteins in MCF-7 live cells. Finally, we used our probe to label CSPs under insulin treatment, revealing several cell surface targets for key functional biomarkers and the pathogenesis of insulin-stimulated MCF-7 breast cancer cells. The above results show that the SPE method provides a promising tool for selectively labelling cell surface proteins and monitoring transient cell surface events.
HostingRepositoryiProX
AnnounceDate2023-08-11
AnnouncementXMLSubmission_2024-01-02_20:00:22.517.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuhao An
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-13 20:14:43ID requested
12024-01-02 20:00:23announced
Publication List
Dai C, Guo X, Pan Z, Wan C, Yang D, Li Y, Lian C, An Y, Zhang T, Yang F, Zhu L, Yin F, Wang R, Li Z, Pyridinium-Based Strategy for a Bioorthogonal Conjugation-Assisted Purification Method for Profiling Cell Surface Proteome. Anal Chem, 95(46):17125-17134(2023) [pubmed]
Keyword List
submitter keyword: Cell surface proteins (CSPs), Sulfo-Pyridinium-Ester (SPE) Probe, Live Cell Labeling, Insulin-Stimulated Pathogenesis
Contact List
Rui Wang
contact affiliationPingshan translational medicine center, Shenzhen Bay Laboratory
contact emailwangrui@szbl.ac.cn
lab head
Yuhao An
contact affiliationPingshan translational medicine center, Shenzhen Bay Laboratory
contact emailanchiyuhao@vip.qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI