PXD044541

PXD044541 is an original dataset announced via ProteomeXchange.

Title	PTMC_A pyridinium-based strategy for bioorthogonal conjugation-assisted purification method for profiling cell surface proteome
Description	Cell surface proteins (CSPs) are valuable targets for therapeutic agents, but achieving highly selective CSP enrichment in cellular physiology remains a technical challenge. To address this challenge, we propose a newly developed sulfo-pyridinium-ester (SPE) cross-linking probe, followed by two-step imaging and enrichment. The SPE probe shows higher efficiency in labelling proteins at the level of cell lysates than similar NHS esters and demonstrates specificity for Lys in competitive experiments. More importantly, this probe can selectively label cell membranes in cell imaging, while only negligible labelling of the intracellular compartment was detected. Moreover, we successfully performed this strategy to mainly label the cell surface proteins in MCF-7 live cells. Finally, we used our probe to label CSPs under insulin treatment, revealing several cell surface targets for key functional biomarkers and the pathogenesis of insulin-stimulated MCF-7 breast cancer cells. The above results show that the SPE method provides a promising tool for selectively labelling cell surface proteins and monitoring transient cell surface events.
HostingRepository	iProX
AnnounceDate	2023-08-11
AnnouncementXML	Submission_2024-01-02_20:00:22.517.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhao An
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-13 20:14:43	ID requested
⏵ 1	2024-01-02 20:00:23	announced

Dai C, Guo X, Pan Z, Wan C, Yang D, Li Y, Lian C, An Y, Zhang T, Yang F, Zhu L, Yin F, Wang R, Li Z, Pyridinium-Based Strategy for a Bioorthogonal Conjugation-Assisted Purification Method for Profiling Cell Surface Proteome. Anal Chem, 95(46):17125-17134(2023) [pubmed]

submitter keyword: Cell surface proteins (CSPs), Sulfo-Pyridinium-Ester (SPE) Probe, Live Cell Labeling, Insulin-Stimulated Pathogenesis

Rui Wang
contact affiliation	Pingshan translational medicine center, Shenzhen Bay Laboratory
contact email	wangrui@szbl.ac.cn
lab head
Yuhao An
contact affiliation	Pingshan translational medicine center, Shenzhen Bay Laboratory
contact email	anchiyuhao@vip.qq.com
dataset submitter

iProX dataset URI