PXD044541
PXD044541 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | PTMC_A pyridinium-based strategy for bioorthogonal conjugation-assisted purification method for profiling cell surface proteome |
Description | Cell surface proteins (CSPs) are valuable targets for therapeutic agents, but achieving highly selective CSP enrichment in cellular physiology remains a technical challenge. To address this challenge, we propose a newly developed sulfo-pyridinium-ester (SPE) cross-linking probe, followed by two-step imaging and enrichment. The SPE probe shows higher efficiency in labelling proteins at the level of cell lysates than similar NHS esters and demonstrates specificity for Lys in competitive experiments. More importantly, this probe can selectively label cell membranes in cell imaging, while only negligible labelling of the intracellular compartment was detected. Moreover, we successfully performed this strategy to mainly label the cell surface proteins in MCF-7 live cells. Finally, we used our probe to label CSPs under insulin treatment, revealing several cell surface targets for key functional biomarkers and the pathogenesis of insulin-stimulated MCF-7 breast cancer cells. The above results show that the SPE method provides a promising tool for selectively labelling cell surface proteins and monitoring transient cell surface events. |
HostingRepository | iProX |
AnnounceDate | 2023-08-11 |
AnnouncementXML | Submission_2024-01-02_20:00:22.517.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuhao An |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-08-13 20:14:43 | ID requested | |
⏵ 1 | 2024-01-02 20:00:23 | announced |
Publication List
Dai C, Guo X, Pan Z, Wan C, Yang D, Li Y, Lian C, An Y, Zhang T, Yang F, Zhu L, Yin F, Wang R, Li Z, Pyridinium-Based Strategy for a Bioorthogonal Conjugation-Assisted Purification Method for Profiling Cell Surface Proteome. Anal Chem, 95(46):17125-17134(2023) [pubmed] |
Keyword List
submitter keyword: Cell surface proteins (CSPs), Sulfo-Pyridinium-Ester (SPE) Probe, Live Cell Labeling, Insulin-Stimulated Pathogenesis |
Contact List
Rui Wang | |
---|---|
contact affiliation | Pingshan translational medicine center, Shenzhen Bay Laboratory |
contact email | wangrui@szbl.ac.cn |
lab head | |
Yuhao An | |
contact affiliation | Pingshan translational medicine center, Shenzhen Bay Laboratory |
contact email | anchiyuhao@vip.qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |