PXD044533

PXD044533 is an original dataset announced via ProteomeXchange.

Title	Molecular characterisation of the Atlantic salmon gill epithelial cell line ASG-10
Description	Fish gills are not only the respiratory organ, but also essential for ion-regulation, acid-base control, detoxification, waste excretion and host defense. Multifactorial gill diseases are common in farmed Atlantic salmon, and still poorly understood. Understanding gill pathophysiology is of paramount importance, but the sacrifice of large numbers of experimental animals for this purpose should be avoided. Therefore, in vitro models, such as cell lines, are urgently required to replace fish trials. An Atlantic salmon gill epithelial cell line, ASG-10, was established at the Norwegian Veterinary institute in 2018. This cell line forms a monolayer expressing cytokeratin, e-cadherin and desmosomes, hallmarks of a functional epithelial barrier. To determine the value of ASG-10 for comparative studies of gill functions, the characterization of ASG-10 was taken one step further by performing functional assays and comparing the cell proteome and transcriptome with those of gills from juvenile freshwater Atlantic salmon. The ASG-10 cell line appear to be a homogenous cell line consisting of epithelial cells, which express tight junction proteins. We demonstrated that ASG-10 forms a barrier, both alone and in co-culture with the Atlantic salmon gill fibroblast cell line ASG- 13. ASG-10 cells can phagocytose and express several ATP-binding cassette transport proteins. Additionally, ASG-10 expresses genes involved in biotransformation of xenobiotics and immune responses. Taken together, this study provides an overview of functions that can be studied using ASG-10, which will be an important contribution to in vitro gill epithelial research of Atlantic salmon.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:03:13.448.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Orla Slattery
SpeciesList	scientific name: Salmo salar (Atlantic salmon); NCBI TaxID: 8030;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-13 14:54:19	ID requested
⏵ 1	2024-01-26 06:03:14	announced

10.3389/fmolb.2023.1242879;

Slattery O, Dahle MK, Sundaram AYM, Nowak BF, Gjessing MC, Solhaug A, gill research. Front Mol Biosci, 10():1242879(2023) [pubmed]

submitter keyword: transcriptome, gill cell line, salmon cell line, proteome

Orla Slattery
contact affiliation	Marine and Freshwater Research Centre Atlantic Technological University, Galway City Campus Ireland
contact email	orla.slattery@atu.ie
lab head
Orla Slattery
contact affiliation	Atlantic Technological University
contact email	orla.slattery@gmit.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044533
     1. Label: PRIDE project
     2. Name: Molecular characterisation of the Atlantic salmon gill epithelial cell line ASG-10