PXD044530

PXD044530 is an original dataset announced via ProteomeXchange.

Title	Distinct phases of cellular signaling revealed by time-resolved protein synthesis
Description	The post-translational regulation of protein function is involved in most cellular processes. As such, synthetic biology tools that operate at this level provide opportunities for manipulating cellular states. Here, we deploy a proximity-triggered protein trans-splicing technology to enable the time-resolved synthesis of target proteins from pre-made parts. The modularity of the strategy allows for the addition or removal of various control elements as a function of the splicing reaction, in the process permitting the cellular location and/or activity state of starting materials and products to be differentiated. The approach is applied to a diverse set of proteins, including the kinase oncofusions BCR/ABL and DNAJB1/PRKACA where dynamic cellular phosphorylation events are dissected, revealing distinct phases of signaling and identifying molecular players connecting the oncofusion to cancer transformation as novel therapeutic targets of cancer cells. We envision that the tools and control strategies developed herein will allow the activity of both naturally occurring and designer proteins to be harnessed for basic and applied research.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:25:31.434.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gihoon Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-13 06:15:24	ID requested
⏵ 1	2025-10-05 16:25:32	announced

10.1038/s41589-024-01677-3;

Lee G, Muir TW, Distinct phases of cellular signaling revealed by time-resolved protein synthesis. bioRxiv, ():(2023) [pubmed]

Lee G, Muir TW, Distinct phases of cellular signaling revealed by time-resolved protein synthesis. Nat Chem Biol, 20(10):1353-1360(2024) [pubmed]

10.1101/2023.07.10.548208;

submitter keyword: Signaling, SILAC AP-MS, Protein engineering, PRM, TMT-phosphoproteomics, Fusion oncogenes

Tom W. Muir
contact affiliation	Department of Chemistry, Princeton University
contact email	muir@princeton.edu
lab head
Gihoon Lee
contact affiliation	Princeton University
contact email	gihoonlee@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD044530

PRIDE project URI

• PRIDE
  1. PXD044530
     1. Label: PRIDE project
     2. Name: Distinct phases of cellular signaling revealed by time-resolved protein synthesis