PXD044512

PXD044512 is an original dataset announced via ProteomeXchange.

Title	A cytoplasmic chemoreceptor and Reactive Oxygen Species mediate bacterial chemotaxis to copper
Description	Chemotaxis is a widespread strategy used by unicellular and multicellular living organisms to maintain their fitness in stressful environments. We previously showed that bacteria can trigger a negative chemotactic response to a copper (Cu)-rich environment. Cu ions toxicity on bacterial cell physiology has been mainly linked to mismetallation events and ROS production, although the precise role of Cu-generated ROS remains largely debated. Here, we found that the cytoplasmic Cu ions content mirrors variations of the extracellular Cu ions concentration and triggers a dose-dependent oxidative stress, which can be abrogated by superoxide dismutase and catalase overexpression. The inhibition of ROS production in the cytoplasm not only improves bacterial growth but also impedes Cu-chemotaxis, indicating that ROS derived from cytoplasmic Cu ions mediate the control of bacterial chemotaxis to Cu. We also identified the Cu chemoreceptor McpR, which binds Cu ions with low affinity, suggesting a labile interaction. In addition, we demonstrate that the cysteine 75 and histidine 99 within the McpR sensor domain are key residues in Cu chemotaxis and Cu coordination. Finally, we discovered that in vitro both Cu(I) and Cu(II) ions modulate McpR conformation in a distinct manner. Overall,our study provides mechanistic insights on a redox-based control of Cu chemotaxis, indicating that the cellular redox status can play a key role in bacterial chemotaxis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:14:30.074.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044512
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Caulobacter vibrioides; NCBI TaxID: 155892;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-11 07:14:45	ID requested
1	2023-09-05 07:30:09	announced
⏵ 2	2023-11-14 07:14:30	announced	2023-11-14: Updated project metadata.

10.6019/PXD044512;

submitter keyword: reactive oxygen species (ROS), stress response, bacteria,Chemotaxis, copper

Prof. Jean-Yves MATROULE
contact affiliation	Research Unit in Microorganisms Biology (URBM), Narilis Institute, University of Namur, Namur, Belgium.
contact email	jean-yves.matroule@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044512
     1. Label: PRIDE project
     2. Name: A cytoplasmic chemoreceptor and Reactive Oxygen Species mediate bacterial chemotaxis to copper