PXD044487

PXD044487 is an original dataset announced via ProteomeXchange.

Title	BtcB (BP2265) is a T3SS chaperone of the CesT family in Bordetella pertussis
Description	Bordetella pertussis is a Gram-negative, strictly human respiratory pathogen and the causative agent of whooping cough (pertussis). Similar to other Gram-negative pathogens, B. pertussis produces a functional type III secretion system, but its role in pathogenesis of B. pertussis is enigmatic and has not yet been elucidated. Here, we applied omics RNA-seq as well as LC-MS/MS techniques and co-immunoprecipitation method to identify and characterize the novel CesT family T3SS chaperone BP2265. Our results show that the chaperone BP2265 specifically interacts with the secreted T3SS anti-sigma factor BtrA. Moreover, in the absence of the chaperone, secretion but not production of BtrA and several early, intermediate, and late T3SS substrates is severely impaired. It appears that the role of BtrA in regulating T3SS is more complex and extends beyond its activity as an antagonist of the sigma factor BtrS. We propose to rename BP2265 as BtcB for the Bordetella type III chaperone of BtrA.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:02:14.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denisa Petrackova
SpeciesList	scientific name: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); NCBI TaxID: 257313; scientific name: Bordetella pertussis B1917; NCBI TaxID: 743277;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-10 12:43:10	ID requested
⏵ 1	2024-01-26 06:02:15	announced

10.1080/22221751.2023.2272638;

Dr, ž, m, í, š, ek J, Petr, á, č, kov, á D, Dienstbier A, Č, urnov, á I, Ve, č, erek B, . Emerg Microbes Infect, 12(2):2272638(2023) [pubmed]

submitter keyword: LC-MSMS, T3SS,Bordetella pertussis, RNA-seq

Branislav Vecerek, PhD
contact affiliation	Czech Academy of Sciences Institute of Microbiology Laboratory of Post-Transcriptional Control of Gene Expression
contact email	vecerek@biomed.cas.cz
lab head
Denisa Petrackova
contact affiliation	Institute of Microbiology of the CAS, v. v. i.
contact email	petrack@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD044487

PRIDE project URI

• PRIDE
  1. PXD044487
     1. Label: PRIDE project
     2. Name: BtcB (BP2265) is a T3SS chaperone of the CesT family in Bordetella pertussis