PXD044482

PXD044482 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoproteomic analysis supporting structural basis for GABAA receptor pi activation
Description	GABAA receptors (GABAAR) are pentameric chloride channels comprising various combinations of different subunits that are activated by γ-aminobutyric acid (GABA), the main inhibitory neurotransmitter in vertebrates. we show here that GABRP chloride channel activity and ERK signaling is directly regulated by GABA binding to GABRP homopentamers. To help uncover GABRP’s link to ERK activity we used phosphoproteomic analysis to reveal signaling pathways regulated by GABA stimulation. Interestingly, and similar to metabotropic GABABRs, GABRP activation leads to phosphorylation of G-protein coupled pathways. These studies open new avenues for understanding GABRP signaling and additionally establish a foundation for conceptualizing the gating mechanism of related pentameric ligand gated ion channels. These studies open new avenues for understanding GABRP signaling and additionally establish a foundation for conceptualizing the gating mechanism of related pentameric ligand gated ion channels.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_17:32:16.743.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenxue Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-10 09:01:28	ID requested
⏵ 1	2026-01-04 17:32:17	announced

Publication List

Wang Y, Zhang Y, Li W, Salovska B, Zhang J, Li T, Li H, Liu Y, Kaczmarek LK, Pusztai L, Klein DE, forms channels that stimulate ERK through a G-protein-dependent pathway. Mol Cell, 85(1):166-176.e5(2025) [pubmed]
10.1016/j.molcel.2024.11.016;

Wang Y, Zhang Y, Li W, Salovska B, Zhang J, Li T, Li H, Liu Y, Kaczmarek LK, Pusztai L, Klein DE, forms channels that stimulate ERK through a G-protein-dependent pathway. Mol Cell, 85(1):166-176.e5(2025) [pubmed]

10.1016/j.molcel.2024.11.016;

Keyword List

submitter keyword: Phosphoproteomics, DIA,GABAA receptor

submitter keyword: Phosphoproteomics, DIA,GABAA receptor

Contact List

yansheng liu
contact affiliation	yale cancer biology institute
contact email	yansheng.liu@yale.edu
lab head
Wenxue Li
contact affiliation	Yale University
contact email	wenxue.li@yale.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD044482
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD044482

PRIDE project URI

Repository Record List

[ + ]