PXD044463

PXD044463 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Digestion of protein substrates by 20S proteasomes
Description	The proteasome is the central protease in generating peptide repertoires for antigen presentation on human leukocyte antigen class I (HLA-I). Proteasomes not only can catalyze peptide hydrolysis but are also capable of mediating transpeptidation, resulting in spliced peptides generation, a process known as proteasome-catalyzed peptide splicing (PCPS). Despite its immunological importance, it is still unclear what are the determinants of PCPS that favor transpeptidation over hydrolysis. Hence, we sought to characterize the processing of many protein substrates and their peptide products by using a simplistic in vitro antigen processing system and analyzed by mass spectrometry followed by a refined downstream data analysis pipeline.
HostingRepository	MassIVE
AnnounceDate	2023-12-22
AnnouncementXML	Submission_2023-12-22_02:05:32.416.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	waitucksoh
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive HF; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-09 01:39:37	ID requested
⏵ 1	2023-12-22 02:05:32	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Mass Spectrometry, antigen processing, 20S proteasomes, MHC-I presentation

submitter keyword: Mass Spectrometry, antigen processing, 20S proteasomes, MHC-I presentation

Contact List

Michele Mishto
contact affiliation	King's College London and the Francis Crick Institute
contact email	michele.mishto@kcl.ac.uk
lab head
Dr. Juliane Liepe
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	juliane.liepe@mpinat.mpg.de
lab head
waitucksoh
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	waituck.soh@mpinat.mpg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092631/