PXD044454

PXD044454 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiles of Human Arterioles Isolated from Fresh Adipose Tissue or Following Overnight Storage
Description	Arterioles are key determinants of the total peripheral vascular resistance, which, in turn, is a key determinant of arterial blood pressure. However, the amount of protein available from one isolated human arteriole may be less than 5µg, making proteomic analysis challenging. In addition, obtaining human arterioles requires manual dissection of unfrozen clinical specimens. This limits its feasibility, especially for powerful multi-center clinical studies in which clinical specimens need to be shipped overnight to a research lab for arteriole isolation. We performed a study to address low input, test overnight tissue storage, and develop a reference human arteriolar proteomic profile. In tandem mass tag proteomics, use of a booster channel consisting of human induced pluripotent stem cells-derived endothelial and vascular smooth muscle cells (1:5 ratio) increased the number of proteins detected in a human arteriole segment with FDR < 0.01 from 1,051 to more than 3,000. The correlation coefficient of proteomic profile was similar between replicate arterioles isolated freshly, following cold storage, or before and after the cold storage (one-way ANOVA, p=0.60). We built a human arteriolar proteomic profile consisting of 3,832 proteins based on the analysis of 12 arteriole samples from three subjects. Of 1,945 BP-relevant proteins that we curated, 476 (12.5%) were detected in the arteriolar proteome, which was a significant overrepresentation (Chi-squared, p<0.05). These findings demonstrate that proteomic analysis is feasible with arterioles isolated from human adipose tissue following cold overnight storage and provide a reference human arteriolar proteome profile highly valuable for studies of arteriole-related traits.
HostingRepository	PRIDE
AnnounceDate	2024-03-21
AnnouncementXML	Submission_2024-03-21_10:55:32.774.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044454
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rajan Pandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-08 11:14:48	ID requested
⏵ 1	2024-03-21 10:55:33	announced

10.1016/J.LABINV.2024.102036;

10.6019/PXD044454;

submitter keyword: Arterioles, TMT labeled Mass Spectrometry, Booster channel, Overnight storage, Proteomics

Mingyu Liang
contact affiliation	Chair, Department of Physiology University of Arizona College of Medicine - Tucson 1656 E Mabel St Tucson, AZ 85721
contact email	mliang1@arizona.edu
lab head
Rajan Pandey
contact affiliation	Post Doc
contact email	rpandey@arizona.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD044454

PRIDE project URI

• PRIDE
  1. PXD044454
     1. Label: PRIDE project
     2. Name: Proteomic Profiles of Human Arterioles Isolated from Fresh Adipose Tissue or Following Overnight Storage