PXD044430

PXD044430 is an original dataset announced via ProteomeXchange.

Title	DDHD2, whose mutation causes spastic paraplegia type 54, is cargo receptor for lipophagy
Description	DDHD2 mutation was a common cause of SPG54 disease. While its mechanism of action was unclear, we aimed to comprehensively characterize the DDHD2 interactome networks in cells. To achieve this, we used affinity purification coupled with mass spectrometry (AP-MS) to define the interactome for DDHD2. We transiently transfected FLAG-DDHD2 expression plasmids into 293T cells, and then performed whole cell lysis for FLAG immunoprecipitation followed by mass spectrometry analysis (IP-MS). As a result, we identified components of the autophagy complex that were enriched in the interactome of DDHD2
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_11:38:08.623.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	fei jia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-08 00:12:48	ID requested
⏵ 1	2025-05-06 11:38:09	announced

Jia F, Wang X, Fu Y, Zhao SM, Lu B, Wang C, DDHD2, whose mutations cause spastic paraplegia type 54, enhances lipophagy via engaging ATG8 family proteins. Cell Death Differ, 31(3):348-359(2024) [pubmed]

10.1038/s41418-024-01261-1;

submitter keyword: Lipophage,DDHD2, SPG54

Chengji Wang
contact affiliation	School of Life Sciences, Fudan University, Shanghai 200438
contact email	chenjiwang@fudan.edu.cn
lab head
fei jia
contact affiliation	Fudan University
contact email	doubleice13127@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD044430

PRIDE project URI

• PRIDE
  1. PXD044430
     1. Label: PRIDE project
     2. Name: DDHD2, whose mutation causes spastic paraplegia type 54, is cargo receptor for lipophagy