PXD044423

PXD044423 is an original dataset announced via ProteomeXchange.

Title	Dynamic protein phosphorylation in Streptococcus pyogenes during growth, stationary phase and starvation
Description	The proteome and phosphoproteome of Streptococcus pyogenes M49 was investigated at different growth phases of cultures grown in either rich medium (THY), chemically defined medium without carbon source, or chemically defined medium containing 1% fructose. Of the 815 phosphosites identified, 463 were included in a label-free quantitative analysis of dynamic protein phosphorylation. A small group of phosphorylation events, almost exclusively at threonine residues, was strongest during growth and decreased during stationary phase. These included phosphorylation sites of the PASTA kinase SP-STK and its putative substrates suggesting that the PASTA kinase-dependent processes regulating the cell cycle in related bacteria are also conserved in S. pyogenes. Most phosphorylation events occurred preferentially at serine residues in the stationary growth phase and under starvation conditions. The elucidation of the physiological significance and the responsible kinases of these phosphorylations require further investigations. In addition to phosphorylation events at S/T/Y residues, phosphoglycerylation of lysine (PGK) occurred frequently among the enriched phosphopeptides.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_10:58:29.666.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044423
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stefan Mikkat
SpeciesList	scientific name: Streptococcus pyogenes NZ131; NCBI TaxID: 471876; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	N6-(3-phosphoglyceryl)-L-lysine; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-07 13:37:57	ID requested
⏵ 1	2024-05-22 10:58:30	announced

10.6019/PXD044423;

10.3390/microorganisms12030621;

Mikkat S, Kreutzer M, Patenge N, during Growth, Stationary Phase, and Starvation. Microorganisms, 12(3):(2024) [pubmed]

submitter keyword: growth phases, phosphoproteome, label-free quantification, PASTA kinase,Streptococcus pyogenes M49, proteome

Stefan Mikkat
contact affiliation	Core Facility Proteome Analysis, Rostock University Medical Center, Rostock, Germany
contact email	stefan.mikkat@med.uni-rostock.de
lab head
Stefan Mikkat
contact affiliation	Core Facility Proteome Analysis, University Medicine Rostock, Schillingallee 69, 18057 Rostock
contact email	stefan.mikkat@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044423
     1. Label: PRIDE project
     2. Name: Dynamic protein phosphorylation in Streptococcus pyogenes during growth, stationary phase and starvation