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PXD044423

PXD044423 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic protein phosphorylation in Streptococcus pyogenes during growth, stationary phase and starvation
DescriptionThe proteome and phosphoproteome of Streptococcus pyogenes M49 was investigated at different growth phases of cultures grown in either rich medium (THY), chemically defined medium without carbon source, or chemically defined medium containing 1% fructose. Of the 815 phosphosites identified, 463 were included in a label-free quantitative analysis of dynamic protein phosphorylation. A small group of phosphorylation events, almost exclusively at threonine residues, was strongest during growth and decreased during stationary phase. These included phosphorylation sites of the PASTA kinase SP-STK and its putative substrates suggesting that the PASTA kinase-dependent processes regulating the cell cycle in related bacteria are also conserved in S. pyogenes. Most phosphorylation events occurred preferentially at serine residues in the stationary growth phase and under starvation conditions. The elucidation of the physiological significance and the responsible kinases of these phosphorylations require further investigations. In addition to phosphorylation events at S/T/Y residues, phosphoglycerylation of lysine (PGK) occurred frequently among the enriched phosphopeptides.
HostingRepositoryPRIDE
AnnounceDate2024-05-22
AnnouncementXMLSubmission_2024-05-22_10:58:29.666.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD044423
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterStefan Mikkat
SpeciesList scientific name: Streptococcus pyogenes NZ131; NCBI TaxID: 471876; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationListN6-(3-phosphoglyceryl)-L-lysine; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-07 13:37:57ID requested
12024-05-22 10:58:30announced
Publication List
10.6019/PXD044423;
10.3390/microorganisms12030621;
Mikkat S, Kreutzer M, Patenge N, during Growth, Stationary Phase, and Starvation. Microorganisms, 12(3):(2024) [pubmed]
Keyword List
submitter keyword: growth phases, phosphoproteome, label-free quantification, PASTA kinase,Streptococcus pyogenes M49, proteome
Contact List
Stefan Mikkat
contact affiliationCore Facility Proteome Analysis, Rostock University Medical Center, Rostock, Germany
contact emailstefan.mikkat@med.uni-rostock.de
lab head
Stefan Mikkat
contact affiliationCore Facility Proteome Analysis, University Medicine Rostock, Schillingallee 69, 18057 Rostock
contact emailstefan.mikkat@med.uni-rostock.de
dataset submitter
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Dataset FTP location
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