PXD044413

PXD044413 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes to immune and inflammatory processes underlie lung preservation using ex vivo cytokine adsorption
Description	The field of graft preservation has made considerable strides in recent years improving outcomes related to solid organ restoration and regeneration. In lungs, the use of ex vivo lung perfusion (EVLP) in line with devices and treatments has shown promising results within preclinical and clinical studies with the potential to improve graft quality. The benefit of the therapy would be to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. Additionally, such therapies used in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when applied to the EVLP circuit and post transplantation. The mechanism by which this treatment improves the donor lung on a molecular basis is not yet fully elucidated. We hypothesized that there were characteristic inflammatory and immunomodulatory differences between lungs treated with and without cytokine adsorption, reflecting in proteomic changes in gene ontology pathways and across inflammation-related proteins. In the current study we investigate the molecular mechanisms and signaling pathways of how cytokine adsorption impacts the lung function when used during EVLP and when used post transplantation as hemoperfusion in a porcine model. Lung tissue from EVLP and post lung transplantation were analyzed for their proteomic profile using mass spectrometry. The inflammatory and immune processes were compared between the treated and the non-treated groups to show the differences occurring between the forms of graft preservation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:34.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Hirdman
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-07 08:24:48	ID requested
1	2023-10-24 10:34:53	announced
⏵ 2	2023-11-14 09:06:43	announced	2023-11-14: Updated project metadata.

Niroomand A, Hirdman G, Pierre L, Ghaidan H, Kjellstr, ö, m S, Stenlo M, Hyll, é, n S, Olm F, Lindstedt S, cytokine adsorption. Front Cardiovasc Med, 10():1274444(2023) [pubmed]

submitter keyword: EVLP, lung transplantation, Cytokine adsorption

Sandra Lindstedt
contact affiliation	Clinical and Experimental Lung Transplantation, Lund University, Sweden
contact email	sandra.lindstedt@med.lu.se
lab head
Gabriel Hirdman
contact affiliation	Lund University
contact email	gabriel.hirdman@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD044413

PRIDE project URI

• PRIDE
  1. PXD044413
     1. Label: PRIDE project
     2. Name: Proteomic changes to immune and inflammatory processes underlie lung preservation using ex vivo cytokine adsorption