PXD044391

PXD044391 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide abundance profiling of yeast deletion strains for GET pathway members using sample multiplexing
Description	The GET pathway is associated with post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER) in yeast, as well as other eukaryotes. Moreover, dysfunction of the GET pathway has been associated with various pathological conditions. In this study, we used yeast deletion strains of Get complex members (specifically, Get1, Get2, Get3, Get4, and Get5) coupled with sample multiplexing-based quantitative mass spectrometry to profile protein abundance on a proteome-wide scale across five individual deletion strains. Our dataset consists of over 4,500 proteins, which corresponds to >75% of the yeast proteome. The data reveal several dozen proteins that are differentially abundant in one or more deletion strains, some of which are membrane-associated, yet the abundance of many TA proteins remained unchanged. This study provides valuable insights into the roles of these Get genes, and the potential for alternative pathways which help maintain cellular function despite the disruption of the GET pathway.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:51.646.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-05 10:15:31	ID requested
1	2023-12-28 11:16:48	announced
⏵ 2	2024-10-22 06:18:52	announced	2024-10-22: Updated project metadata.

10.1002/pmic.202300303;

Gygi JS, Liu X, Levi BP, Paulo JA, Proteome-wide abundance profiling of yeast deletion strains for GET pathway members using sample multiplexing. Proteomics, 24(17):e2300303(2024) [pubmed]

submitter keyword: FAIMS, TMTpro, RTS-MS3,GET pathway, isobaric tagging

Joao A. Paulo
contact affiliation	Harvard Medical School Department of Cell Biology Harvard Medical School Boston, MA, USA
contact email	joao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD044391

PRIDE project URI

• PRIDE
  1. PXD044391
     1. Label: PRIDE project
     2. Name: Proteome-wide abundance profiling of yeast deletion strains for GET pathway members using sample multiplexing