PXD044380

PXD044380 is an original dataset announced via ProteomeXchange.

Title	• YjgM is a novel crotonyltransferase critical for polymyxin resistance of Escherichia coli
Description	Lysine crotonylation has attracted widespread attention in recent years. However, little is known about bacterial crotonylation, particularly crotonyltransferase and decrotonylase, and how it affects antibiotic resistance. Our study demonstrated the ubiquitous presence of crotonylation in E.coli and its connection to bacterial resistance to polymyxin. We first identified the crotonyltransferase YjgM and its regulatory pathways in E. coli with a focus on crotonylation. Further studies suggested that YjgM upregulates the crotonylation of the substrate protein PmrA, which is crucial for controlling E. coli's resistance to polymycin, thereby boosting PmrA's affinity for binding to the promoter of eptA, which in turn promotes EptA expression and confers polymyxin resistance in E. coli. Additionally, we discovered that PmrA's crucial crotonylation site and functional site is Lys 164. These significant discoveries highlight the role of crotonylation in bacterial drug resistance and offer a fresh perspective for creating antibacterial medicines.
HostingRepository	PRIDE
AnnounceDate	2024-03-19
AnnouncementXML	Submission_2024-03-18_20:32:52.054.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhuang jianpeng
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	crotonylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-05 00:29:02	ID requested
⏵ 1	2024-03-18 20:32:52	announced

Dataset with its publication pending

submitter keyword: crotonylation, crotonyltransferase, YjgM, polymyxin resistance, E.coli

Jianpeng Zhuang
contact affiliation	Jinan University
contact email	1047996365@qq.com
lab head
zhuang jianpeng
contact affiliation	jinan university
contact email	1047996365@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044380
     1. Label: PRIDE project
     2. Name: • YjgM is a novel crotonyltransferase critical for polymyxin resistance of Escherichia coli