PXD044379

PXD044379 is an original dataset announced via ProteomeXchange.

Title	The ER-SURF pathway uses ER-mitochondria contact sites for protein targeting to mitochondria
Description	Most mitochondrial proteins are synthesized on cytosolic ribosomes and imported into mitochondria in a post-translational reaction. Mitochondrial precursor proteins which use the ER-SURF pathway employ the surface of the endoplasmic reticulum (ER) as an important sorting platform. How they reach the mitochondrial import machinery from the ER is not known. Here we show that mitochondrial contact sites play a crucial role in the ER-to-mitochondria transfer of precursor proteins. The ER encounter structure (ERMES) and Tom70 are part of two cooperative and partially redundant ER-to-mitochondria transfer routes. If the ER-to-mitochondria transfer is prevented, many mitochondrial precursor proteins accumulate non-productively on the ER surface, resulting in mitochondrial dysfunction. Our observations support an active role of the ER in mitochondrial protein biogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-03-05
AnnouncementXML	Submission_2024-03-05_00:12:49.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-04 21:47:17	ID requested
⏵ 1	2024-03-05 00:12:49	announced

10.1038/S44319-024-00113-W;

submitter keyword: Contact sites. Endoplasmic reticulum. ERMES. ER-SURF. Protein import. Protein targeting. Mitochondria

Markus Räschle
contact affiliation	Molecular Genetics RPTU Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD044379

PRIDE project URI

• PRIDE
  1. PXD044379
     1. Label: PRIDE project
     2. Name: The ER-SURF pathway uses ER-mitochondria contact sites for protein targeting to mitochondria