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PXD044344

PXD044344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInteractome of P21 Activated Kinase 1 (PAK1) in oligodendrocytes
DescriptionIn the central nervous system (CNS), myelin formation by oligodendrocytes (OLs) relies on actin dynamics. Actin polymerization supports the ensheathment step, when the OL process contacts and surrounds a selected portion of axon. While a drastic shift to actin depolymerization is then required to enable the wrapping process and the formation of a multilayered myelin sheath. Molecular mechanisms regulating this switch to actin depolymerization are not fully elucidated. P21-activated kinase 1 (PAK1), a downstream effector of Rho GTPases Rac1 and Cdc42, highly expressed in OLs, can regulate actin dynamics through its kinase activity. We found that PAK1 loss-of-function in OLs, in vivo, stimulates the wrapping and the thickening of myelin sheaths. We showed that PAK1 is highly expressed in myelinating OLs, yet in its inactive form, the one with the ability to trigger actin disassembly. Interestingly, we found that modulations of PAK1 kinase activity control actin dynamics in OLs, thereby myelin formation. Our data demonstrate that PAK1 inactivation in OLs is required for actin depolymerization and proper myelin formation, suggesting the presence of an endogenous PAK1 inhibitor in myelinating OLs. Proteomic analysis of PAK1 binding partners enabled us to identify several proteins likely to regulate its activity.
HostingRepositoryPRIDE
AnnounceDate2024-06-03
AnnouncementXMLSubmission_2024-06-03_01:15:15.324.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCédric Pionneau
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-04 00:08:23ID requested
12024-06-03 01:15:15announced
Publication List
10.1002/GLIA.24570;
Keyword List
submitter keyword: mass spectrometry, oligodendrocyte,PAK1 (P21 Activated Kinase 1), timsTOF PRO
Contact List
Lamia BOUSLAMA-OUEGHLANI
contact affiliationParis Brain Institute – ICM, Sorbonne Université, Inserm, CNRS, AP-HP, Hôpital Pitié-Salpêtrière, Paris, France
contact emaillamia.bouslama@sorbonne-universite.fr
lab head
Cédric Pionneau
contact affiliationPlateforme P3S UMS-37 PASS – Sorbonne Universite
contact emailcedric.pionneau@sorbonne-universite.fr
dataset submitter
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Dataset FTP location
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