PXD044344

PXD044344 is an original dataset announced via ProteomeXchange.

Title	Interactome of P21 Activated Kinase 1 (PAK1) in oligodendrocytes
Description	In the central nervous system (CNS), myelin formation by oligodendrocytes (OLs) relies on actin dynamics. Actin polymerization supports the ensheathment step, when the OL process contacts and surrounds a selected portion of axon. While a drastic shift to actin depolymerization is then required to enable the wrapping process and the formation of a multilayered myelin sheath. Molecular mechanisms regulating this switch to actin depolymerization are not fully elucidated. P21-activated kinase 1 (PAK1), a downstream effector of Rho GTPases Rac1 and Cdc42, highly expressed in OLs, can regulate actin dynamics through its kinase activity. We found that PAK1 loss-of-function in OLs, in vivo, stimulates the wrapping and the thickening of myelin sheaths. We showed that PAK1 is highly expressed in myelinating OLs, yet in its inactive form, the one with the ability to trigger actin disassembly. Interestingly, we found that modulations of PAK1 kinase activity control actin dynamics in OLs, thereby myelin formation. Our data demonstrate that PAK1 inactivation in OLs is required for actin depolymerization and proper myelin formation, suggesting the presence of an endogenous PAK1 inhibitor in myelinating OLs. Proteomic analysis of PAK1 binding partners enabled us to identify several proteins likely to regulate its activity.
HostingRepository	PRIDE
AnnounceDate	2024-06-03
AnnouncementXML	Submission_2024-06-03_01:15:15.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cédric Pionneau
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-04 00:08:23	ID requested
⏵ 1	2024-06-03 01:15:15	announced

10.1002/GLIA.24570;

submitter keyword: mass spectrometry, oligodendrocyte,PAK1 (P21 Activated Kinase 1), timsTOF PRO

Lamia BOUSLAMA-OUEGHLANI
contact affiliation	Paris Brain Institute – ICM, Sorbonne Université, Inserm, CNRS, AP-HP, Hôpital Pitié-Salpêtrière, Paris, France
contact email	lamia.bouslama@sorbonne-universite.fr
lab head
Cédric Pionneau
contact affiliation	Plateforme P3S UMS-37 PASS – Sorbonne Universite
contact email	cedric.pionneau@sorbonne-universite.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD044344

PRIDE project URI

• PRIDE
  1. PXD044344
     1. Label: PRIDE project
     2. Name: Interactome of P21 Activated Kinase 1 (PAK1) in oligodendrocytes