PXD044341

PXD044341 is an original dataset announced via ProteomeXchange.

Title	Overexpression of protein import machinery subunit ycf1/Tic214 represses photosynthetic complex biogenesis by inhibiting the synthesis of plastid-encoded core subunits in tobacco
Description	ycf1 is a chloroplast-encoded large open reading frame. Recently, the Ycf1 protein was identified as a component (Tic214) of the translocon complex in the inner envelope membrane of chloroplasts (TIC). Here, by construction of transplastomic ycf1 overexpression (OE) lines in tobacco, we show that the protein level of Ycf1 is tightly regulated. Slight overaccumulation of the Ycf1 protein leads to strongly inhibited translation of chloroplast-encoded core subunits of the photosynthetic complexes, thus causing a massive repression of photosystem biogenesis and photosynthetic capacity. The nucleus-encoded subunits of the photosystems, especially the light-harvesting complex (LHC) proteins, are much less affected and promote the formation of hyperstacked grana thylakoids in transplastomic ycf1 OE lines. Our data indicate that Ycf1 participates in the control of photosystem biogenesis by directly modulating the translation of the core subunits of photosynthetic complexes in the chloroplast, or alternatively, by affecting the client selectivity of the protein import machinery.
HostingRepository	PRIDE
AnnounceDate	2025-07-16
AnnouncementXML	Submission_2025-07-15_18:24:40.987.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yinsong Wang
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-03 23:10:00	ID requested
⏵ 1	2025-07-15 18:24:41	announced

Dataset with its publication pending

submitter keyword: thylakoid ultrastructure,ycf1, photosystem biogenesis, translation

Guo-Zhang Wu
contact affiliation	Shanghai Jiao Tong University
contact email	gzwu@sjtu.edu.cn
lab head
Yinsong Wang
contact affiliation	Shanghai Jiao Tong University
contact email	020150910029@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044341
     1. Label: PRIDE project
     2. Name: Overexpression of protein import machinery subunit ycf1/Tic214 represses photosynthetic complex biogenesis by inhibiting the synthesis of plastid-encoded core subunits in tobacco