PXD044326 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Antibody Characterisation using FFAP |
Description | Modern therapeutic approaches, especially for cancer and certain autoimmune disorders, evolved and have started to frequently rely on monoclonal antibodies (mAb) or other recombinantly prepared biomolecules (biopharmaceuticals) instead of on small molecules. The mAb treatment is effective but comes at a high financial cost. That is why there is a high demand to develop and market so-called ‘biosimilars’, which are highly similar to already approved biopharmaceuticals.The major hindrance for biosimilars manufacturers is that they must declare and prove an almost identical structure, biological activity, quality, safety and efficacy that apply to the original innovator molecule. To prove structural identity, it’s necessary to characterize not only primary sequences and post-translational modification of expressed proteins but also proper protein folding, and any chemical modifications introduced by production, purification and long-term storage. To determine higher order structure of antibodies, a broad palette of techniques can be considered. In this project we used recently discovered fast fluor-alkylation of proteins (FFAP) that offers another approach for structural characterization of therapeutic antibodies and identifying epitope-paratope interacting regions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-21 |
AnnouncementXML | Submission_2024-05-21_14:03:05.386.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zdenek Kukacka |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF SCP |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-03 09:09:57 | ID requested | |
⏵ 1 | 2024-05-21 14:03:06 | announced | |
Publication List
Keyword List
submitter keyword: covalent labeling, protein structure, mass spectrometry, FFAP,Togni agents |
Contact List
Petr Novak |
contact affiliation | Institute of Microbiology, The Czech Academy of Scicences |
contact email | pnovak@biomed.cas.cz |
lab head | |
Zdenek Kukacka |
contact affiliation | Institute of Microbiology, The Czech Academy of Sciences |
contact email | zdenek.kukacka@biomed.cas.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044326
- Label: PRIDE project
- Name: Antibody Characterisation using FFAP