PXD044326

PXD044326 is an original dataset announced via ProteomeXchange.

Title	Antibody Characterisation using FFAP
Description	Modern therapeutic approaches, especially for cancer and certain autoimmune disorders, evolved and have started to frequently rely on monoclonal antibodies (mAb) or other recombinantly prepared biomolecules (biopharmaceuticals) instead of on small molecules. The mAb treatment is effective but comes at a high financial cost. That is why there is a high demand to develop and market so-called ‘biosimilars’, which are highly similar to already approved biopharmaceuticals.The major hindrance for biosimilars manufacturers is that they must declare and prove an almost identical structure, biological activity, quality, safety and efficacy that apply to the original innovator molecule. To prove structural identity, it’s necessary to characterize not only primary sequences and post-translational modification of expressed proteins but also proper protein folding, and any chemical modifications introduced by production, purification and long-term storage. To determine higher order structure of antibodies, a broad palette of techniques can be considered. In this project we used recently discovered fast fluor-alkylation of proteins (FFAP) that offers another approach for structural characterization of therapeutic antibodies and identifying epitope-paratope interacting regions.
HostingRepository	PRIDE
AnnounceDate	2024-05-21
AnnouncementXML	Submission_2024-05-21_14:03:05.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zdenek Kukacka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-03 09:09:57	ID requested
⏵ 1	2024-05-21 14:03:06	announced

Fojt, í, k L, Kalaninov, á Z, Fiala J, Halada P, Chmel, í, k J, Man P, Kuka, č, ka Z, Nov, á, k P, Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting. Anal Chem, 96(19):7386-7393(2024) [pubmed]

10.1021/acs.analchem.3c04161;

submitter keyword: covalent labeling, protein structure, mass spectrometry, FFAP,Togni agents

Petr Novak
contact affiliation	Institute of Microbiology, The Czech Academy of Scicences
contact email	pnovak@biomed.cas.cz
lab head
Zdenek Kukacka
contact affiliation	Institute of Microbiology, The Czech Academy of Sciences
contact email	zdenek.kukacka@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044326

PRIDE project URI

• PRIDE
  1. PXD044326
     1. Label: PRIDE project
     2. Name: Antibody Characterisation using FFAP