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PXD044326

PXD044326 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAntibody Characterisation using FFAP
DescriptionModern therapeutic approaches, especially for cancer and certain autoimmune disorders, evolved and have started to frequently rely on monoclonal antibodies (mAb) or other recombinantly prepared biomolecules (biopharmaceuticals) instead of on small molecules. The mAb treatment is effective but comes at a high financial cost. That is why there is a high demand to develop and market so-called ‘biosimilars’, which are highly similar to already approved biopharmaceuticals.The major hindrance for biosimilars manufacturers is that they must declare and prove an almost identical structure, biological activity, quality, safety and efficacy that apply to the original innovator molecule. To prove structural identity, it’s necessary to characterize not only primary sequences and post-translational modification of expressed proteins but also proper protein folding, and any chemical modifications introduced by production, purification and long-term storage. To determine higher order structure of antibodies, a broad palette of techniques can be considered. In this project we used recently discovered fast fluor-alkylation of proteins (FFAP) that offers another approach for structural characterization of therapeutic antibodies and identifying epitope-paratope interacting regions.
HostingRepositoryPRIDE
AnnounceDate2024-05-21
AnnouncementXMLSubmission_2024-05-21_14:03:05.386.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZdenek Kukacka
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF SCP
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-03 09:09:57ID requested
12024-05-21 14:03:06announced
Publication List
Fojt, í, k L, Kalaninov, á Z, Fiala J, Halada P, Chmel, í, k J, Man P, Kuka, č, ka Z, Nov, á, k P, Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting. Anal Chem, 96(19):7386-7393(2024) [pubmed]
10.1021/acs.analchem.3c04161;
Keyword List
submitter keyword: covalent labeling, protein structure, mass spectrometry, FFAP,Togni agents
Contact List
Petr Novak
contact affiliationInstitute of Microbiology, The Czech Academy of Scicences
contact emailpnovak@biomed.cas.cz
lab head
Zdenek Kukacka
contact affiliationInstitute of Microbiology, The Czech Academy of Sciences
contact emailzdenek.kukacka@biomed.cas.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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