PXD044325

PXD044325 is an original dataset announced via ProteomeXchange.

Title	Effect of an immune challenge and feed supplements on broiler chicken individual breast muscle protein synthesis
Description	Optimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken growth. Despite of its importance, research on broiler chicken muscle protein dynamics has been mostly limited to the study of mixed protein turnover. The present study aims to characterize the effect of a bacterial challenge and the feed supplementation of a citrus and a cucumber extract on broiler chicken individual breast muscle proteins fractional synthesis rates (FSR) using a recently developed dynamic proteomics pipeline. 21 day-old broiler chickens were administered a single 2H2O dose before being culled at different timepoints. A total of 60 breast muscle protein extracts from five experimental groups (Unchallenged, Challenged, Control Diet, Diet 1 and Diet 2) were analyzed using a DDA proteomics approach. Proteomics data was filtered in order to reliably calculate multiple proteins FSR making use of a newly developed bioinformatics pipeline. Broiler breast muscle proteins FSR uniformly decreased following a bacterial challenge, this change was judged significant for 15 individual proteins, the two major functional clusters identified as well as for mixed breast muscle protein. Citrus or cucumber extract feed supplementation did not show any effect on the breast muscle protein FSR of immunologically challenged broilers. The present study has identified potential predictive markers of breast muscle growth and provided new information on broiler chicken breast muscle protein turnover which could be essential for improving the efficiency of broiler chicken meat production.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:10.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduardo Zarzuela
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-03 08:05:51	ID requested
1	2024-04-04 04:22:19	announced
⏵ 2	2024-10-22 06:35:11	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2024.105158;

Peinado-Izaguerri J, Corbishley A, Zarzuela E, Pina-Beltr, á, n B, Riva F, McKeegan DEF, Bain M, Mu, ñ, oz J, Bhide M, McLaughlin M, Preston T, Effect of an immune challenge and two feed supplements on broiler chicken individual breast muscle protein synthesis rate. J Proteomics, 299():105158(2024) [pubmed]

submitter keyword: chicken, citrus, FSR, muscle

Javier Muñoz
contact affiliation	CNIO
contact email	javier.munozperalta@osakidetza.eus
lab head
Eduardo Zarzuela
contact affiliation	Centro Nacional de Investigaciones Oncológicas
contact email	eduzarfer@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044325
     1. Label: PRIDE project
     2. Name: Effect of an immune challenge and feed supplements on broiler chicken individual breast muscle protein synthesis