PXD044317

PXD044317 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of differential cellular genome and proteome regulation by HIV-1 and HIV-2 pseudovirions in the early phase of infection
Description	Beside the similar structure and genomic organization of human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2), striking difference exist between the in terms of replication dynamics and clinical manifestation of infection. Although pathomechanism of HIV-1 infection well characterized, relatively few data are available regarding HIV-2 viral replication, and its interaction with host cell protein during the early phase of infection. We utilized proteo-transcriptomic analyses to determine differential genome expression and proteomic changes induced by transduction with HIV-1 / 2 pseudovirions during eight, 12, and 26 hour time-points in HEK-293T cells. We show that alteration in the cellular milieu was indeed different between the two pseudovirions. The significantly higher number of genes altered by HIV-2 in the first two time-points, suggest a more diverse, yet subtle effect on the host cell, preparing the infected cell for integration and latency. On the other hand, GO analysis showed that, while HIV-1 induced cellular oxidative stress and had a greater effect on the cellular metabolism, HIV-2 mostly affected genes involved in cell adhesion, extracellular matrix organization or keratinocyte differentiation. Proteomics analysis revealed that HIV-1 upregulated, while HIV-2 downregulated proteins involved viral protein expression and replication. Our study provides an insight into the understudied replication cycle of HIV-2, and enrich our knowledge about the use of HIV-based lentiviral vectors in general.
HostingRepository	MassIVE
AnnounceDate	2024-01-16
AnnouncementXML	Submission_2024-01-16_04:21:13.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gergő Kall�
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human immunodeficiency virus 1; NCBI TaxID: 11676; scientific name: Human immunodeficiency virus 2; NCBI TaxID: 11709;
ModificationList	Oxidation; Carbamidomethyl; Acetyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-03 04:12:46	ID requested
⏵ 1	2024-01-16 04:21:18	announced

Linkner TR, Ambrus V, Kunkli B, Szojka ZI, Kall, ó G, Cs, ő, sz É, Kumar A, Emri M, T, ő, zs, é, r J, Mahdi M, Comparative Analysis of Differential Cellular Transcriptome and Proteome Regulation by HIV-1 and HIV-2 Pseudovirions in the Early Phase of Infection. Int J Mol Sci, 25(1):(2023) [pubmed]

submitter keyword: HIV1, HIV2, proteomics

Gergo Kallo
contact affiliation	University of Debrecen
contact email	kallo.gergo@med.unideb.hu
lab head
Gergő Kall�
contact affiliation	University of Debrecen
contact email	kallo.gergo@med.unideb.hu
dataset submitter

MassIVE dataset URI

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