PXD044296

PXD044296 is an original dataset announced via ProteomeXchange.

Title	Proximity proteomics using AMPL-MS coupled to analysis for histone modification
Description	Eukaryotic chromatin is organised in individual domains, which are defined by their location within the nuclear space and their distinct proteomic compositions. In contrast to cellular organelles surrounded by lipid membranes, the composition of distinct chromatin domains is rather ill described and considered to be highly dynamic. To identify proteins associated with a specific chromatin domain and to better understand how those domains are established and maintained, we established AMPL-MS (Antibody mediated proximity labelling mass spectrometry). As this method is based on antibodies and does not require the expression of fusion proteins, it constitutes a versatile and very sensitive method to characterize chromatin domains containing specific signature proteins or histone modifications. We used AMPL-MS to characterize the chromocenter as well as the chromosome territory containing the hyperactive X-chromosome in Drosophila. Our data revealed the presence of many RNA binding proteins in these domains thereby underscoring the importance of nuclear RNA in the organisation of the genome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:04.513.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Axel Imhof
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monomethylated residue; trimethylated residue; acetylated residue; dimethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-02 05:03:55	ID requested
1	2024-05-29 06:24:34	announced
⏵ 2	2024-10-22 06:43:04	announced	2024-10-22: Updated project metadata.

10.7554/ELIFE.95718;

submitter keyword: histone modification, centromere, Protein A-Apex2, X-chromosome, Biotinylation, chromatin,Proximity proteomics

Prof Axel Imhof
contact affiliation	Ludwig-Maximilians-Universität München Biomedical Center Munich Molecular Biology Großhadernerstr. 9 82152 Planegg-Martinsried
contact email	imhof@lmu.de
lab head
Axel Imhof
contact affiliation	BioMedical Center
contact email	imhof@lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044296

PRIDE project URI

• PRIDE
  1. PXD044296
     1. Label: PRIDE project
     2. Name: Proximity proteomics using AMPL-MS coupled to analysis for histone modification