PXD044294

PXD044294 is an original dataset announced via ProteomeXchange.

Title	A simple method for developing lysine targeted covalent protein reagents
Description	In this project we developed phosphopeptides functionalized with methacrylate ester warheads installed on a cysteine residue, which bind covalently to the protein 14-3-3 sigma. We characterized the binding of these peptides to 14-3-3 proteins in A549 lysates as well as off-targets. We prepared biotinylated forms of the 14-3-3 binding peptides and incubated A549 lysates with these peptides, followed by Streptavidin pull down, trypsin digestion and LCMSMS. All isoforms of 14-3-3 were pull downed as well as multiple proteins with phosphate-containing substrates such as NAD-dependent enzymes.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:03:26.797.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ronen Gabizon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-02 03:28:47	ID requested
⏵ 1	2024-01-26 06:03:28	announced

10.1038/s41467-023-42632-5;

Gabizon R, Tivon B, Reddi RN, van den Oetelaar MCM, Amartely H, Cossar PJ, Ottmann C, London N, A simple method for developing lysine targeted covalent protein reagents. Nat Commun, 14(1):7933(2023) [pubmed]

submitter keyword: peptides,Covalent probes

Nir London
contact affiliation	Department of Chemical and Structural Biology, Weizmann Institute, Rehovot, Israel
contact email	nir.london@weizmann.ac.il
lab head
Ronen Gabizon
contact affiliation	Department of Chemical and Structural Biology, Weizmann Institute of Science
contact email	ronen.gabizon@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD044294

PRIDE project URI

• PRIDE
  1. PXD044294
     1. Label: PRIDE project
     2. Name: A simple method for developing lysine targeted covalent protein reagents