PXD044293

PXD044293 is an original dataset announced via ProteomeXchange.

Title	In vitro and In vivo Antimicrobial Potential of Lithium Complex against Multi Drug Resistant Acinetobacter baumannii
Description	Acinetobacter baumannii is a Gram-negative ESKAPE pathogen, accountable for lethal nosocomial infections, owing to its high pathogenicity and multi-drug resistance (MDR). Hence, the World Health Organization (WHO) has prioritized this bacterium against which new pharmacophores are required urgently. Therefore, this study evaluates the antimicrobial potential of lithium complex based on salicylic acid, and 1,10-phenanthroline ([Li(phen)2sal]) against MDR A. baumannii (MDRAB) through growth inhibition, time-kill assay, biofilm inhibition, and eradication alongside microscopy. The mechanism of action was proposed by flow cytometry and proteomic analysis. Moreover, its in-vivo efficacy was assessed using A. baumannii-induced pneumonia model in mice. Results showed that lithium complex significantly inhibited the bacterial growth at 16-32 µg/ml. Time kill analysis revealed that the compound retarded the bacterial growth after 2-3 h of treatment. Lithium complex significantly inhibited and eradicated >70% of biofilms at the MIC level which is a key problem of indwelling and prosthetic devices. Light and atomic force microscopy further indicated disarticulated biofilm and ruptured cellular membrane post-lithium complex treatment. Proteomic analysis revealed that lithium complex exerted oxidative stress on A. baumannii which may contribute to cell rupturing. Flow cytometry confirmed the role of lithium complex in cytoplasmic membrane disintegration. The compound ameliorated the pneumonia symptoms at non-toxic doses of 20 and 40 mg/kg in the pre-clinical A. baumannii-induced pneumonia model as evident by the significant reduction in bacterial burden and parenchymal inflammation. Taken together, these findings suggest that the lithium complex can be a potential drug candidate against MDR A. baumannii.
HostingRepository	PRIDE
AnnounceDate	2023-09-22
AnnouncementXML	Submission_2023-09-22_00:12:26.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MunazzaRaza
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Acinetobacter baumannii; NCBI TaxID: 470;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-02 03:28:00	ID requested
⏵ 1	2023-09-22 00:12:27	announced

Dataset with its publication pending

ProteomeXchange project tag: Human Proteome Project

submitter keyword: lithium complex, nosocomial infections, Acinetobacter baumannii, metal complexes, multi drug resistant, biofilm

MunazzaRaza
contact affiliation	Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
contact email	munzihyder@yahoo.com
lab head
MunazzaRaza
contact affiliation	International center for chemical and biological sciences
contact email	munzihyder@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044293
     1. Label: PRIDE project
     2. Name: In vitro and In vivo Antimicrobial Potential of Lithium Complex against Multi Drug Resistant Acinetobacter baumannii