PXD044282

PXD044282 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MORAIS_SynapticMitoReplication
Description	In neurons it is assumed mitochondrial replication only occurs in the cell body and then mitochondria have to travel to the periphery. Although mitochondrial replication has been observed to occur away from the cell body, the mechanisms involved are still elusive. Using EdU-labelling in mouse primary neurons, we developed a tool to determine the mitochondrial replication rate. Taking of advantage of microfluidic devices, we confirmed that mitochondrial replication also occurs locally in the periphery of neurons. To achieve this, mitochondria require de novo nuclear-encoded, but not mitochondrial-encoded protein translation. Following a proteomic screen comparing synaptic with non-synaptic mitochondria we identified two elongation factors eEF1A1 and TUFM that were upregulated in synaptic mitochondria. We found that mitochondrial replication is impaired upon downregulation of eEF1A1, and this is particularly relevant in the periphery of neurons.
HostingRepository	MassIVE
AnnounceDate	2023-08-02
AnnouncementXML	Submission_2023-08-02_07:16:03.247.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeffrey N. Savas
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-01 21:42:54	ID requested
⏵ 1	2023-08-02 07:16:03	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Mitochondria, Synapse

submitter keyword: Mitochondria, Synapse

Contact List

Jeffrey N. Savas, PhD
contact affiliation	Department of Neurology Northwestern University
contact email	jeffrey.savas@northwestern.edu
lab head
Jeffrey N. Savas
contact affiliation	Northwestern University
contact email	jeffrey.savas@northwestern.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092568/