PXD044270 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic Analysis of Diabetic Retinas (proteoglycomics data only) |
Description | As a metabolic disease, diabetes often leads to health complications such as heart failure, nephropathy, neurological disorders, and vision loss. Diabetic retinopathy (DR) affects as many as 100 million people worldwide. The mechanism of DR is complex and known to impact both neural and vascular components in the retina. While recent advances in the field have identified major cellular signaling contributing to DR pathogenesis, little has been reported on the protein post-translational modifications (PTM) -known to define protein localization, function, and activity -in the diabetic retina overall. Protein glycosylation is the enzymatic addition of carbohydrates to proteins, which can influence many protein attributes including folding, stability, function, and subcellular localization. Olinked glycosylation is the addition of sugars to an oxygen atom in amino acids with a free oxygen atom in their side chain (i.e., threonine, serine). To date, more than 100 congenital disorders of glycosylation have been described. However, no studies have identified the retinal O-linked glycoproteome in health or disease. With a critical need to expedite the discovery of PTMomics in diabetic retinas, we identified both global changes in protein levels and the retinal O-glycoproteome of control and diabetic mice. Liquid chromatography/mass spectrometry-based glycoproteomics and high throughput screening identified proteins differentially glycosylated in the retinas of wildtype and diabetic mice. Here, we provide evidence that diabetes shifts O-glycosylation of metabolic and synaptic proteins in the retina. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:00:28.179.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044270 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | james mobley |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-01 12:02:28 | ID requested | |
1 | 2023-08-15 16:28:45 | announced | |
⏵ 2 | 2024-10-22 06:00:28 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: mouse, proteomics, retina, diabetes mellitus |
Contact List
James A. Mobley, Ph.D. |
contact affiliation | Director, UAB CCC Proteomics Facility; Professor, UAB Anetsthesiology |
contact email | mobleyja@uab.edu |
lab head | |
james mobley |
contact affiliation | University of Alabama at Birmingham |
contact email | mobleyja@uab.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD044270 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044270
- Label: PRIDE project
- Name: Proteomic Analysis of Diabetic Retinas (proteoglycomics data only)