PXD044262
PXD044262 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Acetic acid is superior to formic acid as an acidifier in sub-nanogram and single cell proteomics |
| Description | This work follows on Battellino et al., 2023 which described the use of 0.5% acetic acid in shotgun proteomics providing additional relative signal over 0.1% formic acid. In this study we performed repeated experiments with K562 serial dilutions down to 20 picogram on column with alternating between these two buffers on an EasyNLC 1200 instrument. We consistently obtain higher numbers of precursors, peptides and proteins when this is the only alteration in the study. In addition we find that with the extra signal obtained when using acetic acid, we can reduce the cycle time of each experiment allowing us to obtain more measurements across each peak at concentrations as low as 100 picogram peptide load on column using a default diaPASEF method on the TIMSTOF SCP. These experiments were repeated with single cells from two cancer cell lines with the same results. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-19 |
| AnnouncementXML | Submission_2024-04-19_10:40:15.568.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ben |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | timsTOF SCP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-08-01 05:57:24 | ID requested | |
| ⏵ 1 | 2024-04-19 10:40:16 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Single cell proteomics, Sub nanogram, single cell, diaPASEF |
Contact List
| Benjamin E. Wolfe | |
|---|---|
| contact affiliation | Tufts University |
| contact email | benjamin.wolfe@tufts.edu |
| lab head | |
| Benjamin C Orsburn | |
| contact affiliation | Johns Hopkins |
| contact email | borsbur1@jhmi.edu |
| lab head | |
| ben | |
| contact affiliation | The Johns Hopkins School of Medicine |
| contact email | ben_orsburn3@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092563/ |
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