PXD044257

PXD044257 is an original dataset announced via ProteomeXchange.

Title	A simple method for developing lysine targeted covalent protein reagents
Description	In this project we developed phosphopeptides functionalized with methacrylate ester warheads installed on a cysteine residue, which bind covalently to the protein 14-3-3 sigma. We prepared complexes of the purified protein with the peptides and used trypsin digestion and LC-MSMS to elucidate the binding site of the peptides, by comparison of compound-treated complexes to DMSO-treated protein and observing the disappearance on non-modified peptides. The file names are based on internal names used for the project and are distinct from the names given to the peptides in the final publication. Names are as follows: 4IEA - peptide 3 in the final publication 128C - peptide 8 in the final publication 132C - peptide 11 in the final publication
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:25:11.911.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044257
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ronen Gabizon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-01 04:29:54	ID requested
1	2024-01-26 06:11:10	announced
⏵ 2	2024-10-22 06:25:18	announced	2024-10-22: Updated project metadata.

10.1038/s41467-023-42632-5;

Gabizon R, Tivon B, Reddi RN, van den Oetelaar MCM, Amartely H, Cossar PJ, Ottmann C, London N, A simple method for developing lysine targeted covalent protein reagents. Nat Commun, 14(1):7933(2023) [pubmed]

10.6019/PXD044257;

submitter keyword: peptides,Covalent probes

Nir London
contact affiliation	Department of Chemical and Structural Biology, Weizmann Institute, Rehovot, Israel
contact email	nir.london@weizmann.ac.il
lab head
Ronen Gabizon
contact affiliation	Department of Chemical and Structural Biology, Weizmann Institute of Science
contact email	ronen.gabizon@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD044257

PRIDE project URI

• PRIDE
  1. PXD044257
     1. Label: PRIDE project
     2. Name: A simple method for developing lysine targeted covalent protein reagents