PXD044240

PXD044240 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans
Description	Assembly of the DNA helicase known as CMG (CDC45-MCM-GINS) is the key regulated step during DNA replication initiation in eukaryotes. Using the Caenorhabditis elegans embryo as a model system, we identify a new CMG assembly factor called DNSN-1, which associates with the BRCT-domain protein MUS-101. We show that DNSN-1 is required to recruit the GINS complex to chromatin and find that DNSN-1 positions GINS on the MCM-2-7 helicase motor, by direct binding of DNSN-1 to GINS and MCM-3, on interfaces that are important for initiation and essential for viability.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:39.609.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044240
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	KARIM LABIB
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-31 16:19:08	ID requested
1	2023-10-24 12:30:51	announced
⏵ 2	2023-11-14 09:09:42	announced	2023-11-14: Updated project metadata.

Publication List

Xia Y, Sonneville R, Jenkyn-Bedford M, Ji L, Alabert C, Hong Y, Yeeles JTP, Labib KPM, . Science, 381(6664):eadi4932(2023) [pubmed]
10.6019/PXD044240;

Xia Y, Sonneville R, Jenkyn-Bedford M, Ji L, Alabert C, Hong Y, Yeeles JTP, Labib KPM, . Science, 381(6664):eadi4932(2023) [pubmed]

10.6019/PXD044240;

Keyword List

submitter keyword: DONSON,C. elegans, Meier Gorlin Syndrome, MUS-101, DNSN-1, CMG helicase, DNA replication

submitter keyword: DONSON,C. elegans, Meier Gorlin Syndrome, MUS-101, DNSN-1, CMG helicase, DNA replication

Contact List

Karim Labib
contact affiliation	MRC Protein Phosphorylation & Ubiquitylation Unit, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland - UK
contact email	kpmlabib@dundee.ac.uk
lab head
KARIM LABIB
contact affiliation	University of Dundee
contact email	kpmlabib@dundee.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD044240
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD044240

PRIDE project URI

Repository Record List

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